April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Tear Cytokine Profile in Medically Treated Glaucoma Patients Undergoing Trabeculectomy: Effect of MCP-1 on Early Post-Operative Outcome
Author Affiliations & Notes
  • R. S. Chong
    Singapore National Eye Centre, Singapore, Singapore
  • Y. Jiang
    Singapore Eye Research Institute, Singapore, Singapore
  • P. Boey
    Singapore National Eye Centre, Singapore, Singapore
  • S. Yu
    Singapore Eye Research Institute, Singapore, Singapore
  • H. Htoon
    Singapore National Eye Centre, Singapore, Singapore
  • T. Aung
    Singapore National Eye Centre, Singapore, Singapore
  • P. Khaw
    NIHR Biomedical Research Centre, Moorfields Eye Hospital, United Kingdom
  • T. Wong
    Singapore National Eye Centre, Singapore, Singapore
  • Footnotes
    Commercial Relationships  R.S. Chong, None; Y. Jiang, None; P. Boey, None; S. Yu, None; H. Htoon, None; T. Aung, None; P. Khaw, None; T. Wong, None.
  • Footnotes
    Support  NMRC:R551/39/2007
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 6125. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      R. S. Chong, Y. Jiang, P. Boey, S. Yu, H. Htoon, T. Aung, P. Khaw, T. Wong; Tear Cytokine Profile in Medically Treated Glaucoma Patients Undergoing Trabeculectomy: Effect of MCP-1 on Early Post-Operative Outcome. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6125.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To determine the tear cytokine profile from medicated glaucoma patients scheduled for trabeculectomy and to establish whether a specifically elevated cytokine level is related to early post-operative scarring.

Methods: : Sixty-one patients treated with topical anti-glaucoma medications and 29 normal subjects with no prior topical treatment were recruited for the study. Schirmer strips were used to collect tear samples. Multiplex bead assay was used to quantify the presence of pro-inflammatory cytokines in the tears. The patients were followed up for six months after surgery to determine whether any post-operative intervention to maintain filtering bleb function was required. The level of cytokines in tear specimens from medicated glaucoma patients was the main outcome measure for the study. The need for post-operative bleb needling within six months was a secondary outcome measure.

Results: : Of the 17 cytokines assayed, only monocyte chemoattractant protein 1 (MCP-1) was significantly elevated in the medicated compared to the non-medicated eyes, (P<0.0004). At 6 months post-op, 18 of the 61 (30%) eyes required post-operative intervention. A much higher MCP-1 level was detected in these eyes compared to the remaining 43 that did not require intervention (P<0.0001). The duration of use of topical medication correlated with increasing levels of MCP-1 although the types of glaucoma medication and the number of bottles of medications did not have any significant relationship with MCP-1.

Conclusions: : MCP-1 was found to be the predominant cytokine elevated in tears from topically medicated glaucoma eyes in an Asian population. Eyes with a propensity to scar in the early post-operative period have a significantly raised level of MCP-1.

Keywords: wound healing • cell-cell communication • drug toxicity/drug effects 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×