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S. Chakrabarty, B. Liu, H. Sen, M. Hu, S. Gordon, R. B. Nussenblatt; Role of the Macrophage Scavenger Receptor in the Laser-Induced Experimental Choroidal Neovascularization (CNV) Mouse Model. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6134.
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The presence of macrophages in the laser induced CNV animal model suggests that these cells play an important role in that disease. The scavenger receptors (SR) comprise a large family of structurally diverse transmembrane cell surface glycoproteins restricted to macrophages, dendritic cells, endothelial cells and a few other cell types. SRs have been demonstrated to play an important role in innate immune defence. We used the Class-A scavenger receptor knock out (SRA-/- and Marco-/-) mice toevaluate the role of the scavenger receptor in the laser-induced experimentalCNV model.
Laser photocoagulation was used to induce CNVs inwild-type C57BL/6J mice or SRA-/- or Marco -/- mice. Eyes were collected atday 10 after laser injury and immunostaining of the eye-cup was done.Multiplane z-series were collected with a confocal microscope and CNVresponses were compared by the volumetric measurements of the blood vesselsusing Volocity image analysis software.
Data to date showed that the development of experimentally induced CNV wasinhibited in SRA-/- mice as compared to the wild type control mice. In contrast the Marco -/- mouse did not show a significant difference when compared with control mice.
Our data suggest that the class-A scavenger receptor might play a role in the formation CNV.
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