Abstract
Purpose: :
The presence of macrophages in the laser induced CNV animal model suggests that these cells play an important role in that disease. The scavenger receptors (SR) comprise a large family of structurally diverse transmembrane cell surface glycoproteins restricted to macrophages, dendritic cells, endothelial cells and a few other cell types. SRs have been demonstrated to play an important role in innate immune defence. We used the Class-A scavenger receptor knock out (SRA-/- and Marco-/-) mice toevaluate the role of the scavenger receptor in the laser-induced experimentalCNV model.
Methods: :
Laser photocoagulation was used to induce CNVs inwild-type C57BL/6J mice or SRA-/- or Marco -/- mice. Eyes were collected atday 10 after laser injury and immunostaining of the eye-cup was done.Multiplane z-series were collected with a confocal microscope and CNVresponses were compared by the volumetric measurements of the blood vesselsusing Volocity image analysis software.
Results: :
Data to date showed that the development of experimentally induced CNV wasinhibited in SRA-/- mice as compared to the wild type control mice. In contrast the Marco -/- mouse did not show a significant difference when compared with control mice.
Conclusions: :
Our data suggest that the class-A scavenger receptor might play a role in the formation CNV.
Keywords: choroid: neovascularization • age-related macular degeneration • inflammation