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R. J. Torres, C. Muccioli, M. Maia, L. Noronha, D. Precoma, A. Luchini, L. A. A. Torres, C. L. A. Torres, R. R. A. Torres, R. A. A. Torres; Decrease of the Immunohistochemical Expression of Calcium Binding Protein (Calretinin) in the Retina of Hypercholesterolemic Rabbits Treated With Roziglitazone. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6148.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate early retinal abnormalities induced by a hypercholesterolemic diet and the prevention of these abnormalities after oral administration of rosiglitazone in rabbits.
54 New Zealand rabbits were divided into four groups: control group, normal diet; G1, hypercholesterolemic diet; G2, hypercholesterolemic diet plus a daily administration of 3 mg rosiglitazone from day 14 on; and G3, hypercholesterolemic diet plus a daily administration of 3 mg rosiglitazone since the start of the experiment. The sclera and choroid underwent histological and histomorphometric analyses and immunohistochemical analysis with Hamm 11 antibody. The retina underwent immunohistochemical analysis with anti-calretinin (intracellular calcium sensor) and GFAP antibodies.
No abnormalities were observed in the control group. G1 had significant increases in scleral and choroidal thicknesses compared with the control group (P=0.008) and group 3 (P<0.05). G1 scleral tissue presented significant immunoreactivity to Hamm 11 marker in relation to the other groups. G1 retina presented significant increases in immunoreactivity (P<0.001) to the anti-calretinin antibody compared with the other groups. G2 and G3 retina had significant (P<0.002) increases in calretinin immunoreactivity compared to control group. GFAP was negative in all groups.
In this experimental model, hypercholesterolemic diet induced early abnormalities in the retina, choroid and sclera. Rosiglitazone preserved the anatomy of these structures.
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