April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Identification of Autoreactive T Cells in Age-Related Macular Degeneration Patients
Author Affiliations & Notes
  • S. S. Huang
    Univeristy Hospitals Eye Institute Dept Ophthalmology and Visual Science,
    Case Western Reserve University, Cleveland, Ohio
  • W. Zou
    Cell Biology, Cleveland Clinic Foundation, Cleveland, Ohio
  • L. Hong
    Chemistry,
    Case Western Reserve University, Cleveland, Ohio
  • R. Salomon
    Chemistry,
    Case Western Reserve University, Cleveland, Ohio
  • J. Liu
    Cell Biology, Cleveland Clinic Foundation, Cleveland, Ohio
  • Footnotes
    Commercial Relationships  S.S. Huang, Digital Healthcare, Inc., I; Second Sight, C; VRT – Vitreo Retinal Technologies – REDIARC, C; American Retina Foundation, C; Diabetic Retinopathy Clinical Research Network (DRCR), C; MacuSight – REDIARC Fundus Reading, C; American Academy of Ophthalmology, C; Alcon – REDIARC Fundus Reading, C; Pfizer – REDIARC Fundus Reading, C; Shering Plough – REDIARC Fundus Reading, C; University Hospitals Eye Institute, E; Therapeutic Nanoparticle and Molecular Imaging, C; SurModics, Inc., I; Philip F. and Elizabeth G. Searle – Suber Huang Chair Professorship, E; Digital Healthcare, Inc., C; i2i Innovative Ideas, Inc., E; American Academy of Family Physicians, C; Retinal Dis Image Analysis Reading Center/Case, C; Bausch & Lomb, C; Neurotech – REDIARC Fundus Reading, C; NEHEP/NEI/NIH, C; Merck & Co., Inc., C; SurModics, Inc., C; Lux Bio – REDIARC Fundus Reading, C; W. Zou, None; L. Hong, None; R. Salomon, None; J. Liu, None.
  • Footnotes
    Support  American Health Assistance Foundation Award M2008-063
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 6198. doi:
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    • Get Citation

      S. S. Huang, W. Zou, L. Hong, R. Salomon, J. Liu; Identification of Autoreactive T Cells in Age-Related Macular Degeneration Patients. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6198.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Carboxyethylpyrrole (CEP) modified proteins are abundant in Bruch’s membrane of AMD eyes and the autoantibodies against CEP adducted proteins are higher in AMD patients. This study is to identify autoreactive T cells in AMD patients.

Methods: : Peripheral blood mononuclear cells (PBMC) were isolated from 20 AMD patients and 20 age-matched controls, and alternatively stimulated with CEP-conjugated human serum albumin (CEP-HSA) or IL2 for six cycles. Cloned CEP-relevant T cell activities were measured by ELISPOT assays or Intracellular cytokine staining. Phenotypes of T cells were characterized by antibody staining followed by flow cytometry. Apoptosis of the cloned T cells was analyzed by mitochondrial membrane potential detection assays.

Results: : The number of the CEP relevant T cell clones was significantly increased in AMD patients compared to the age-matched controls. The cloned AMD patient T cells demonstrated augmented IFN-γ and interleukin-17 producing cells. Further study revealed that the AMD patient cloned T cells down-regulated the mitochondrial depolarization compared to the cloned T cells from age-matched controls.

Conclusions: : AMD patients showed enhanced T cell activities, which include increased CEP relevant cloned T cells, and IFN-γ and IL-17 producing T cells. The increased number of cloned T cells may result, in part, from the suppressed T cell mitochondrial apoptotic pathway.

Keywords: age-related macular degeneration • immunomodulation/immunoregulation • apoptosis/cell death 
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