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S. S. Huang, W. Zou, L. Hong, R. Salomon, J. Liu; Identification of Autoreactive T Cells in Age-Related Macular Degeneration Patients. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6198.
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© ARVO (1962-2015); The Authors (2016-present)
Carboxyethylpyrrole (CEP) modified proteins are abundant in Bruch’s membrane of AMD eyes and the autoantibodies against CEP adducted proteins are higher in AMD patients. This study is to identify autoreactive T cells in AMD patients.
Peripheral blood mononuclear cells (PBMC) were isolated from 20 AMD patients and 20 age-matched controls, and alternatively stimulated with CEP-conjugated human serum albumin (CEP-HSA) or IL2 for six cycles. Cloned CEP-relevant T cell activities were measured by ELISPOT assays or Intracellular cytokine staining. Phenotypes of T cells were characterized by antibody staining followed by flow cytometry. Apoptosis of the cloned T cells was analyzed by mitochondrial membrane potential detection assays.
The number of the CEP relevant T cell clones was significantly increased in AMD patients compared to the age-matched controls. The cloned AMD patient T cells demonstrated augmented IFN-γ and interleukin-17 producing cells. Further study revealed that the AMD patient cloned T cells down-regulated the mitochondrial depolarization compared to the cloned T cells from age-matched controls.
AMD patients showed enhanced T cell activities, which include increased CEP relevant cloned T cells, and IFN-γ and IL-17 producing T cells. The increased number of cloned T cells may result, in part, from the suppressed T cell mitochondrial apoptotic pathway.
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