April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Effect of Hypertonic Saline on the Immunogenicity of Keratocytes in Porcine Cornea
H. Choi; E. Han; J. Ko; H. Lee; W. Wee; J. Lee; M. Kim
Author Affiliations & Notes
  • H. Choi
    Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Aritificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • E. Han
    Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Aritificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • J. Ko
    Seoul Aritificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • H. Lee
    Seoul Aritificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • W. Wee
    Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Aritificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • J. Lee
    Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Ophthalmology, Seoul National University Bundang Hospital, Seongnam, Republic of Korea
  • M. Kim
    Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Aritificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  H. Choi, None; E. Han, None; J. Ko, None; H. Lee, None; W. Wee, None; J. Lee, None; M. Kim, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 6210. doi:
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      H. Choi, E. Han, J. Ko, H. Lee, W. Wee, J. Lee, M. Kim; Effect of Hypertonic Saline on the Immunogenicity of Keratocytes in Porcine Cornea. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6210.

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Abstract

Purpose: : To investigate effect of hypertonic saline on the immunogenicity of keratocytes in porcine cornea

Methods: : Primary cultivated porcine keratocytes were treated with 1.5 mol sodium chloride solution for 6 hours. Alteration of the expression in swine leukocyte antigen (SLA)I, SLAII, and α-gal was evaluated using flow cytometry (FACS Canto; Becton-Dickinson, Mountain View, Calif, United States) and antigenicity was compared with in no-treated control. Release of high-mobility group box 1 (HMGB1) was analyzed with Western blot to investigate cell damage, and was compared with in ethanol-treated control. Change of immune reaction associated gene expression was assessed in DNA microarray using Platinum pig 13 K oligo chip (GenoCheck Co., Ltd., Ansan, Korea) and real-time PCR was performed for more than 1.5 up- or down-regulated immune related genes.

Results: : Hypertonic saline treated keratocytes showed lower SLAI and SLAII expression and released less amount of HMGB1 compared with those in each control. Among the more than 1.5 up- or down-regulated immune -related genes, real-time PCR corroborated that trombospondin1 and heat shock protein 70kDa protein5 mRNA were increased and cadherin5, matrix metallopeptidase 1, matrix metallopeptidase 2, tissue factor, and plasminogen activator inhibitorI mRNA were diminished in hypertonic saline treated keratocytes.

Conclusions: : Hypertonic saline treatment is likely to mitigate immunogenicity and enhance tissue-protective factors in keratocytes.

Keywords: cornea: stroma and keratocytes • flow cytometry • gene microarray 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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