Purchase this article with an account.
H. Choi, E. Han, J. Ko, H. Lee, W. Wee, J. Lee, M. Kim; Effect of Hypertonic Saline on the Immunogenicity of Keratocytes in Porcine Cornea. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6210.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate effect of hypertonic saline on the immunogenicity of keratocytes in porcine cornea
Primary cultivated porcine keratocytes were treated with 1.5 mol sodium chloride solution for 6 hours. Alteration of the expression in swine leukocyte antigen (SLA)I, SLAII, and α-gal was evaluated using flow cytometry (FACS Canto; Becton-Dickinson, Mountain View, Calif, United States) and antigenicity was compared with in no-treated control. Release of high-mobility group box 1 (HMGB1) was analyzed with Western blot to investigate cell damage, and was compared with in ethanol-treated control. Change of immune reaction associated gene expression was assessed in DNA microarray using Platinum pig 13 K oligo chip (GenoCheck Co., Ltd., Ansan, Korea) and real-time PCR was performed for more than 1.5 up- or down-regulated immune related genes.
Hypertonic saline treated keratocytes showed lower SLAI and SLAII expression and released less amount of HMGB1 compared with those in each control. Among the more than 1.5 up- or down-regulated immune -related genes, real-time PCR corroborated that trombospondin1 and heat shock protein 70kDa protein5 mRNA were increased and cadherin5, matrix metallopeptidase 1, matrix metallopeptidase 2, tissue factor, and plasminogen activator inhibitorI mRNA were diminished in hypertonic saline treated keratocytes.
Hypertonic saline treatment is likely to mitigate immunogenicity and enhance tissue-protective factors in keratocytes.
This PDF is available to Subscribers Only