Purchase this article with an account.
R. R. Loureiro, P. C. Cristovam, C. M. Martins, J. L. Covre, R. M. Hazarbassanov, J. Pereira Gomes; Comparison of Different Culture Media for Limbal Epithelial Cells Cultivated Ex Vivo. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6242.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Evaluate the effectiveness of different culture media on growth, proliferation, apoptosis and differentiation of limbal epithelial cells cultivated ex vivo.
Corneal rims from different donors had their posterior stroma removed and were cultured in three different culture media: SHEM, KSFM, Epilife. The epithelial cell cultures were submitted to analysis of growth and epithelial migration; immunocytochemistry for ABCG2, p63, Ki67, CK3 and VMT; RT-PCR; and cell viability test with Hoechst. All results were statistically compared.
Epithelial cells cultivated in SHEM medium presented rapid and progressive growth, with a highly positive percentage of cells expressing epithelial cytokeratin CK3. The epithelial cells cultivated in KSFM showed epithelial and mesenchymal appearance and high positivity for ABCG2, p63, Ki67 and VMT. A similar pattern of antigen expression was noted with the epithelial cells cultivated in Epilife media. However, in the latter, cells presented only an epithelial phenotype.
We conclude that Epilife and KSFM seem to be the best media for establishing limbal epithelial cell cultures. They contain low calcium concentration and keep the cells in a more undifferentiated status when compared to cells cultured in SHEM medium.
This PDF is available to Subscribers Only