April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Alpha-Crystallins Interact With Caspase-3 and Bax in vitro and in vivo to Prevent Stress-Induced Apoptosis and Cataractogenesis
Author Affiliations & Notes
  • J. Liu
    Biochemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • L. Gong
    Biochemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • M. Deng
    Biochemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • D. Yuan
    Biochemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • L. Zhang
    Biochemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
    College of Life Sciences, Hunan Normal University, Changsha, China
  • S. Sun
    Biochemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • H. Ma
    Biochemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • D. Li
    Biochemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
    Ophthalmology & Visual Sciences, University of Nebraska Medical Center, Omaha, Nebraska
  • Footnotes
    Commercial Relationships  J. Liu, None; L. Gong, None; M. Deng, None; D. Yuan, None; L. Zhang, None; S. Sun, None; H. Ma, None; D. Li, None.
  • Footnotes
    Support  NIH Grant EY18380
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 6362. doi:
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      J. Liu, L. Gong, M. Deng, D. Yuan, L. Zhang, S. Sun, H. Ma, D. Li; Alpha-Crystallins Interact With Caspase-3 and Bax in vitro and in vivo to Prevent Stress-Induced Apoptosis and Cataractogenesis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6362.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Previous studies from our lab and others have shown that alpha-crystallins can regulate members in both caspase and Bcl-2 families to prevent stress-induced apoptosis, which leads to cataractogenesis. However, the exact mechanisms by which alpha-crystallins regulate these members remain uncertain. In the present study, we examined the interactions between alpha-crystallins and Caspase-3 as well as Bax to explore how alpha-crystallins regulate stress-induced apoptosis.

Methods: : Cell flow cytometry and MTT assays were used to detect apoptosis. In vitro binding assay, surface plasmon resonance, co-immunoprecipitation, and yeast two hybrid selection were used to detect the interactions between alpha-crystallins and caspase-3 as well as Bax.

Results: : Both alpha-crystallins interact with caspase-3 and Bax in vitro. AlphaB-crystallin display stronger affinity to caspase-3 than alphaA-crystallin does. The interacting complexes between alpha-crystallins and caspase-3/Bax were detected in both lens epithelial cell lines and also in developing and adult lenses.

Conclusions: : alpha-crystallins directly interact with Caspase-3 as well as Bax to prevent stress-induced apoptosis.

Keywords: crystallins • apoptosis/cell death • cell survival 
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