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M. Ruggeri, O. P. Kocaoglu, S. Uhlhorn, D. Borja, R. Urs, T.-H. Chou, V. Porciatti, J.-M. Parel, F. Manns; In vivo Quantitative Analysis of Ocular Biometric Parameters in the C57BL/6J Mouse. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6363.
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© ARVO (1962-2015); The Authors (2016-present)
To measure the shape and dimensions of the whole mouse eye in vivo using OCT images corrected for distortions due to refraction.
A custom-built OCT system (Vision Research 2008; 48:2732-8) was used to obtain images of the whole mouse eye. We developed a semi-automated segmentation method to detect the boundaries of the anterior and posterior corneal, lens and retinal surfaces as well as the anterior surface of the iris. The radius of curvature and asphericity of the surfaces were calculated using a conic section fit of each boundary. Image distortions due to refraction of the OCT beam at the successive boundaries were corrected using a ray-tracing algorithm. Corrected ocular distances, radii of curvature and asphericities of the cornea and lens surfaces, and anterior chamber angle were obtained on 3 C57BL/6J mice.
Biometric parameters of C57BL/6J mice compared well with previous published data obtained from histological sections. The study demonstrates the feasibility of quantitative in vivo biometry of mouse models.Support: NIH grants R01EY019077, P30-EY14801 (Center grant), NEI 5F31EY15395 (DB), the Florida Lions Eye Bank, unrestricted grant from Research to Prevent Blindness, Inc., Henri and Flore Lesieur Foundation (JMP)
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