April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
In vivo Quantitative Analysis of Ocular Biometric Parameters in the C57BL/6J Mouse
Author Affiliations & Notes
  • M. Ruggeri
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida
  • O. P. Kocaoglu
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida
  • S. Uhlhorn
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida
  • D. Borja
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida
  • R. Urs
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida
  • T.-H. Chou
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida
  • V. Porciatti
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • J.-M. Parel
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida
  • F. Manns
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida
  • Footnotes
    Commercial Relationships  M. Ruggeri, None; O.P. Kocaoglu, None; S. Uhlhorn, None; D. Borja, None; R. Urs, None; T.-H. Chou, None; V. Porciatti, None; J.-M. Parel, None; F. Manns, None.
  • Footnotes
    Support  See above
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 6363. doi:https://doi.org/
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    • Get Citation

      M. Ruggeri, O. P. Kocaoglu, S. Uhlhorn, D. Borja, R. Urs, T.-H. Chou, V. Porciatti, J.-M. Parel, F. Manns; In vivo Quantitative Analysis of Ocular Biometric Parameters in the C57BL/6J Mouse. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6363. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

To measure the shape and dimensions of the whole mouse eye in vivo using OCT images corrected for distortions due to refraction.

 
Methods:
 

A custom-built OCT system (Vision Research 2008; 48:2732-8) was used to obtain images of the whole mouse eye. We developed a semi-automated segmentation method to detect the boundaries of the anterior and posterior corneal, lens and retinal surfaces as well as the anterior surface of the iris. The radius of curvature and asphericity of the surfaces were calculated using a conic section fit of each boundary. Image distortions due to refraction of the OCT beam at the successive boundaries were corrected using a ray-tracing algorithm. Corrected ocular distances, radii of curvature and asphericities of the cornea and lens surfaces, and anterior chamber angle were obtained on 3 C57BL/6J mice.

 
Results:
 

In vivo

 
Conclusions:
 

Biometric parameters of C57BL/6J mice compared well with previous published data obtained from histological sections. The study demonstrates the feasibility of quantitative in vivo biometry of mouse models.Support: NIH grants R01EY019077, P30-EY14801 (Center grant), NEI 5F31EY15395 (DB), the Florida Lions Eye Bank, unrestricted grant from Research to Prevent Blindness, Inc., Henri and Flore Lesieur Foundation (JMP)  

 
Keywords: imaging/image analysis: non-clinical • anterior segment • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) 
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