April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Podoplanin Regulates Corneal Epithelial Morphology
Author Affiliations & Notes
  • K. Maruyama
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo-ku, Japan
  • F. Bock
    Augenklinik Erlangen, SFB 643, Erlangen, Germany
  • U. Schlotzer-Schrehardt
    Department of Ophthalmology, University of Erlangen-Nuremberg, Erlangen, Germany
  • T. Nakamura
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo-ku, Japan
  • D. Kerjaschki
    Pathology, Medical University Vienna, Vienna, Austria
  • F. E. Kruse
    Department of Ophthalmology, University of Erlangen-Nuremberg, Erlangen, Germany
  • S. Kinoshita
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo-ku, Japan
  • C. Cursiefen
    Department of Ophthalmology, University of Erlangen-Nuremberg, Erlangen, Germany
  • Footnotes
    Commercial Relationships  K. Maruyama, None; F. Bock, None; U. Schlotzer-Schrehardt, None; T. Nakamura, None; D. Kerjaschki, None; F.E. Kruse, None; S. Kinoshita, None; C. Cursiefen, None.
  • Footnotes
    Support  KAKENHI 19791283
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 6371. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      K. Maruyama, F. Bock, U. Schlotzer-Schrehardt, T. Nakamura, D. Kerjaschki, F. E. Kruse, S. Kinoshita, C. Cursiefen; Podoplanin Regulates Corneal Epithelial Morphology. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6371.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Podoplanin has been shown to be a reliable marker of lymphatic endothelium. In addition it has previously been shown to be expressed in basal corneal epithelium. Here we investigate a potential novel role of Podoplanin in regulating epithelial cell morphology and cell turnover.

Methods: : Male C57BL/6 mice were used as control and male podoplanin null mice were used for analysis of corneal epithelial thickness. In vitro, we used human cultivated corneal epithelial cell sheets treated with either 1µg/ml human podoplanin Fc for last 3 days in cultivation period or control Fc. The cultivated epithelium was examined by sectional image (HE staining) and electron microscopy (EM).

Results: : Immunohistochemistry demonstrates constitutive expression of Podoplanin in basal human and murine corneal epithelium. Podoplanin null mice had a significantly thicker corneal epithelial layer than control mice. Moreover, administration of Podoplanin Fc in vitro produced a thicker corneal epithelium layer than control Fc.

Conclusions: : Podoplanin seems to be a novel regulator of corneal epithelial morphology and cell turnover. These data functionally implicate Podoplanin in the process of cell proliferation.

Keywords: cornea: epithelium • cornea: basic science • pathology: experimental 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×