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K. Maruyama, F. Bock, U. Schlotzer-Schrehardt, T. Nakamura, D. Kerjaschki, F. E. Kruse, S. Kinoshita, C. Cursiefen; Podoplanin Regulates Corneal Epithelial Morphology. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6371.
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© ARVO (1962-2015); The Authors (2016-present)
Podoplanin has been shown to be a reliable marker of lymphatic endothelium. In addition it has previously been shown to be expressed in basal corneal epithelium. Here we investigate a potential novel role of Podoplanin in regulating epithelial cell morphology and cell turnover.
Male C57BL/6 mice were used as control and male podoplanin null mice were used for analysis of corneal epithelial thickness. In vitro, we used human cultivated corneal epithelial cell sheets treated with either 1µg/ml human podoplanin Fc for last 3 days in cultivation period or control Fc. The cultivated epithelium was examined by sectional image (HE staining) and electron microscopy (EM).
Immunohistochemistry demonstrates constitutive expression of Podoplanin in basal human and murine corneal epithelium. Podoplanin null mice had a significantly thicker corneal epithelial layer than control mice. Moreover, administration of Podoplanin Fc in vitro produced a thicker corneal epithelium layer than control Fc.
Podoplanin seems to be a novel regulator of corneal epithelial morphology and cell turnover. These data functionally implicate Podoplanin in the process of cell proliferation.
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