April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Fish Scale-Derived Biocornea
C. Lin; R. Ritch; S. Lin; M.-H. Ni; Y.-C. Chang; H. Lai; F.-H. Lin
Author Affiliations & Notes
  • C. Lin
    Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan
    Department of Research, Aeon Astron Europe B.V., Leiden, The Netherlands
  • R. Ritch
    Ophthalmology, New York Eye & Ear Infirmary, New York, New York
    Department of Ophthalmology, The New York Medical College, Valhalla, New York
  • S. Lin
    Department of Materials and Textiles, Oriental Institute of Technology, Taipei, Taiwan
  • M.-H. Ni
    Department of Research, Aeon Astron Europe B.V., Leiden, The Netherlands
  • Y.-C. Chang
    Department of Research, Aeon Astron Europe B.V., Leiden, The Netherlands
  • H. Lai
    Department of Research, Aeon Astron Europe B.V., Leiden, The Netherlands
  • F.-H. Lin
    Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan
  • Footnotes
    Commercial Relationships  C. Lin, Aeon Astron Corp., E; priority date: 2006/12/04, P; R. Ritch, Aeon Astron Corp., C; S. Lin, Aeon Astron Corp., I; priority date: 2006/12/04, P; M.-H. Ni, Aeon Astron Corp., E; Y.-C. Chang, Aeon Astron Corp., E; H. Lai, Aeon Astron Corp., I; priority date: 2006/12/04, P; F.-H. Lin, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 6439. doi:
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      C. Lin, R. Ritch, S. Lin, M.-H. Ni, Y.-C. Chang, H. Lai, F.-H. Lin; Fish Scale-Derived Biocornea. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6439.

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Abstract

Purpose: : To develop a novel scaffold, derived from fish scales, as an alternative functional material for corneal regenerative applications.

Methods: : Fish scales were acellularized, decalcified and fabricated into collagen scaffolds. The microstructure of the acellularized scaffold was imaged by scanning electron microscopy (SEM). To assess the cytocompatibility of the scaffold with corneal cells, rabbit corneal cells were cultured on the scaffold and examined under SEM and confocal microscopy at different time periods.

Results: : SEM micrographs revealed a 3-dimensional patterned scaffold with a microchannel-like structure. The acellularization and decalcification treatments did not affect the naturally micropatterned structure of the material. Rapid corneal cell proliferation and migration on the scaffold were observed. Corneal cells proliferated exuberantly and started stacking in the guidance channels on the scaffold surfaces after more than 3 days of culture.

Conclusions: : We provide evidences for the feasibility of the scaffold as a template for corneal cells growth and migration. The fish scale-derived scaffold can be developed as a promising material for tissue-engineering of cornea.

Keywords: cornea: basic science 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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