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C. Lin, R. Ritch, S. Lin, M.-H. Ni, Y.-C. Chang, H. Lai, F.-H. Lin; Fish Scale-Derived Biocornea. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6439.
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© ARVO (1962-2015); The Authors (2016-present)
To develop a novel scaffold, derived from fish scales, as an alternative functional material for corneal regenerative applications.
Fish scales were acellularized, decalcified and fabricated into collagen scaffolds. The microstructure of the acellularized scaffold was imaged by scanning electron microscopy (SEM). To assess the cytocompatibility of the scaffold with corneal cells, rabbit corneal cells were cultured on the scaffold and examined under SEM and confocal microscopy at different time periods.
SEM micrographs revealed a 3-dimensional patterned scaffold with a microchannel-like structure. The acellularization and decalcification treatments did not affect the naturally micropatterned structure of the material. Rapid corneal cell proliferation and migration on the scaffold were observed. Corneal cells proliferated exuberantly and started stacking in the guidance channels on the scaffold surfaces after more than 3 days of culture.
We provide evidences for the feasibility of the scaffold as a template for corneal cells growth and migration. The fish scale-derived scaffold can be developed as a promising material for tissue-engineering of cornea.
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