May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
No Consequence of Dietary Omega-3 Polyunsaturated Fatty Acid Deficiency on the Severity of Scopolamine-Induced Dry Eye
Author Affiliations & Notes
  • S. Viau
    Eye & Nutrition Research Group, National Inst Agronomic Research, Dijon, France
  • B. Pasquis
    Eye & Nutrition Research Group, National Inst Agronomic Research, Dijon, France
  • M.-A. Maire
    Eye & Nutrition Research Group, National Inst Agronomic Research, Dijon, France
  • L. Bretillon
    Eye & Nutrition Research Group, National Inst Agronomic Research, Dijon, France
  • S. Grégoire
    Eye & Nutrition Research Group, National Inst Agronomic Research, Dijon, France
  • N. Acar
    Eye & Nutrition Research Group, National Inst Agronomic Research, Dijon, France
  • A. M. Bron
    Eye & Nutrition Research Group, National Inst Agronomic Research, Dijon, France
    Department of Ophthalmology, University Hospital, Dijon, France
  • C. P. Creuzot-Garcher
    Eye & Nutrition Research Group, National Inst Agronomic Research, Dijon, France
    Department of Ophthalmology, University Hospital, Dijon, France
  • C. Joffre
    Eye & Nutrition Research Group, National Inst Agronomic Research, Dijon, France
  • Footnotes
    Commercial Relationships  S. Viau, None; B. Pasquis, None; M. Maire, None; L. Bretillon, None; S. Grégoire, None; N. Acar, None; A.M. Bron, None; C.P. Creuzot-Garcher, None; C. Joffre, None.
  • Footnotes
    Support  INRA, F-21000 Dijon; Regional Council of Burgundy, F-21000 Dijon
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 118. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      S. Viau, B. Pasquis, M.-A. Maire, L. Bretillon, S. Grégoire, N. Acar, A. M. Bron, C. P. Creuzot-Garcher, C. Joffre; No Consequence of Dietary Omega-3 Polyunsaturated Fatty Acid Deficiency on the Severity of Scopolamine-Induced Dry Eye. Invest. Ophthalmol. Vis. Sci. 2008;49(13):118. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Epidemiological studies suggest that dietary omega-3 polyunsaturated fatty acids (PUFAs) may protect against prevalence of dry eye. This work aimed to evaluate whether a dietary deficiency in omega-3 PUFAs may increase the severity of dry eye in a scopolamine-induced rat model.

Methods: : Three consecutive generations of Lewis rats were bred under diets deprived of omega-3 PUFAs. Dry eye was experimentally induced by continuous scopolamine delivery in female animals from the third deficient generation and in female Lewis rats fed for three generations with a balanced diet. After 14 days of treatment, the clinical signs of ocular dryness were evaluated in vivo using fluorescein staining. MHCII and the mucin Muc5AC were immunostained on ocular sphere cryosections. Lipids were extracted from the exorbital lacrimal glands and phospholipid fatty acids were analyzed by gas chromatography.

Results: : The ratio of fluorescein stained area to total area of the cornea was significantly increased in scopolamine-treated animals when compared to not implanted animals (from 2.4 ± 1.0% to 23.5 ± 13.6% for the balanced diet and from 7.8 ± 6.1% to 20.1 ± 6.2% for the deficient diet). Scopolamine treatment decreased Muc5AC immunostaining in the conjunctival epithelium (-34% for the balanced diet and -23% for the deficient diet) and tended to increase MHCII immunostaining in the conjunctival epithelium for both diets. In exorbital lacrimal gland phospholipids, arachidonic acid (AA) and the delta5-desaturase index (ratio of AA to dihomo gamma-linolenic acid) were significantly increased by scopolamine treatment for both diets. There was no significant diet-difference in scores of fluorescein staining, Muc5AC and MHCII immunostaining. The omega-3 PUFA deficiency induced a significant increase in AA in the exorbital lacrimal gland (+38% for the control animals and +15% for the scopolamine-treated animals).

Conclusions: : Our data suggest that, unexpectedly, an omega-3 PUFA deficiency did not increase the severity of dry eye in the rat.

Keywords: conjunctiva • cornea: epithelium • lipids 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×