May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
The Diabetes Risk Gene Wolframin (WFS1) Is Expressed in Retina
Author Affiliations & Notes
  • R. G. Schmidt-Kastner
    College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida
  • P. Kreczmanski
    Dept. of Psychiatry and Neuropsychology, Maastricht University, Maastricht, The Netherlands
  • H. Yamamoto
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, Univ. Miami Miller School of Medicine, Miami, Florida
  • M. Preising
    Dept. of Ophthalmology, Universitaetsklinikum Giessen und Marburg GmbH, Giessen, Germany
  • R. Diederen
    Dept. of Psychiatry and Neuropsychology, Maastricht University, Maastricht, The Netherlands
  • C. J. Dougherty
    College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida
  • J.-M. Parel
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, Univ. Miami Miller School of Medicine, Miami, Florida
  • C. Schmitz
    Dept. of Psychiatry and Neuropsychology, Maastricht University, Maastricht, The Netherlands
  • J. Blanks
    College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida
  • C. K. Dorey
    College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida
  • Footnotes
    Commercial Relationships  R.G. Schmidt-Kastner, None; P. Kreczmanski, None; H. Yamamoto, None; M. Preising, None; R. Diederen, None; C.J. Dougherty, None; J. Parel, None; C. Schmitz, None; J. Blanks, None; C.K. Dorey, None.
  • Footnotes
    Support  EY016119; Henri and Flore Lesieur Foundation
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 166. doi:https://doi.org/
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      R. G. Schmidt-Kastner, P. Kreczmanski, H. Yamamoto, M. Preising, R. Diederen, C. J. Dougherty, J.-M. Parel, C. Schmitz, J. Blanks, C. K. Dorey; The Diabetes Risk Gene Wolframin (WFS1) Is Expressed in Retina. Invest. Ophthalmol. Vis. Sci. 2008;49(13):166. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The study of rare monogenic disorders can generate novel insight into common disorders such as diabetes. Mutations of WFS1 (wolframin) cause recessive Wolfram syndrome, characterized by diabetes, optic atrophy and deafness. Wolframin is expressed in pancreatic islet cells, and common WFS1 variants have been associated with the risk of type 2 diabetes. We reported expression of wolframin in monkey retinal ganglion cells (RGCs; Yamamoto et al., 2006). This study describes expression of wolframin in human and rat retinas. Additional risk genes for diabetes were searched for expression in the retina in silico.

Methods: : Immunohistochemistry was performed on retinal sections obtained from human donor eyes (with no prior history of diabetes) using an affinity purified polyclonal antibody against the N-terminus of human wolframin (Yamamoto et al., 2006). To analyze expression in RPE, sections of albino rats and (human) ARPE-19 cells were also studied. A list of diabetes risk genes was compiled from current reviews. Retinal mRNA expression data of diabetes risk genes were probed in EyeBank using WFS1 as a reference. Further evidence for retinal expression was retrieved from PubMed.

Results: : Wolframin was localized in human and rat RGCs. The RPE of albino rats and ARPE-19 cells expressed wolframin. Diabetes risk genes listed in EyeBank for expression in the retina and reported in specific studies on the retina included NEUROD1, FTO and ABCC8/SUR1.

Conclusions: : Diabetic retinopathy is commonly interpreted as the consequence of systemic hyperglycemia. Primary retinal dysfunction in diabetes has been also proposed. Expression of wolframin in the human retina, documented here at the protein level, and retinal expression of three additional diabetes risk genes provide a novel, genetic aspect for primary retinal dysfunction in diabetic retinopathy.

Keywords: diabetic retinopathy • gene/expression • immunohistochemistry 
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