May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Spatial Configuration of Neovasculature in Retinoblastoma: A Proposed Model for Tumor Vascular Development
Author Affiliations & Notes
  • Y. Pina
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • H. Boutrid
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • M. E. Jockovich
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • T. G. Murray
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • Footnotes
    Commercial Relationships  Y. Pina, None; H. Boutrid, None; M.E. Jockovich, None; T.G. Murray, None.
  • Footnotes
    Support  NIH R01 EY013629, NIH center grant P30 EY014801 and by an unrestricted grant to the University of Miami from Research to Prevent Blindness, Inc.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 18. doi:https://doi.org/
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      Y. Pina, H. Boutrid, M. E. Jockovich, T. G. Murray; Spatial Configuration of Neovasculature in Retinoblastoma: A Proposed Model for Tumor Vascular Development. Invest. Ophthalmol. Vis. Sci. 2008;49(13):18. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The purpose of this study is to evaluate the spatial distribution of blood vessels in the LHBETATAG mouse model of retinoblastoma. We propose to model tumor vascular development and to correlate this model with proposed impacts on antiangiogenic therapies for the treatment of retinoblastoma.

Methods: : The study protocol was approved by the University of Miami, IACUC. All experiments were conducted in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. Vessel heterogeneity in retinal tumors from LHBETATAG mice of advanced disease 12 and 16 weeks of age (n=8 per group) were assessed by immunofluorescence. Total blood vessels were detected by immunostaining with lectin from bandeira simplicifolia, neovessels with CD105 (endoglin) and pericyte-committed mature vessels with α-smooth muscle actin.

Results: : As previously reported, tumor vasculature is evenly distributed throughout the tumor area. The degree of vessel maturation, however, was heterogeneous among different tumor areas. Tumors had higher mean neovessel densities than mature vasculature at tumor apex (p=0.03) and tumor margins (p=0.04). On the other hand, differences between neovessel and mature vessel densities were not significant (p=0.1). These results suggest that angiogenic activity is mainly present at leading edges of tumors and vessel maturation ensues in areas closest to the tumor base.

Keywords: retina • tumors • immunohistochemistry 
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