May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Antibody Mediated Retinopathy - New Mechanisms and Treatment Strategies
Author Affiliations & Notes
  • S. D. Grozdanic
    Iowa State University, Ames, Iowa
    Veterinary Clinical Sciences,
  • M. M. Harper
    Iowa State University, Ames, Iowa
    Veterinary Clinical Sciences,
  • H. Kecova
    Iowa State University, Ames, Iowa
    Veterinary Clinical Sciences,
  • W. Nilaweera
    Iowa State University, Ames, Iowa
    Veterinary Clinical Sciences,
  • A. E. Boll
    Iowa State University, Ames, Iowa
    Veterinary Clinical Sciences,
  • M. H. Kuehn
    Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa
  • D. S. Sakaguchi
    Iowa State University, Ames, Iowa
    Development and Cell Biology,
    Biomedical Sciences,
  • R. H. Kardon
    Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa
    Veterans Administration Medical Center, Iowa City, Iowa
  • Footnotes
    Commercial Relationships  S.D. Grozdanic, None; M.M. Harper, None; H. Kecova, None; W. Nilaweera, None; A.E. Boll, None; M.H. Kuehn, None; D.S. Sakaguchi, None; R.H. Kardon, None.
  • Footnotes
    Support  VA RRD C3919R and ISU Biotechnology Fund
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 198. doi:https://doi.org/
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      S. D. Grozdanic, M. M. Harper, H. Kecova, W. Nilaweera, A. E. Boll, M. H. Kuehn, D. S. Sakaguchi, R. H. Kardon; Antibody Mediated Retinopathy - New Mechanisms and Treatment Strategies. Invest. Ophthalmol. Vis. Sci. 2008;49(13):198. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To characterize the molecular nature of spontaneous retinopathy (sudden acquired retinal degeneration syndrome-SARDS) in dogs and determine therapeutic effect of intravenous immunoglobulins (IVIg) and systemic steroids on restoration of different visual parameters.

Methods: : Six eyes from 5 SARDS dogs euthanized due to irreversible blindness were analyzed using microarray analysis and immunohistochemistry for presence of immune cells and complement expression. Seven additional dogs diagnosed with SARDS were examined using pupil light reflex (PLR) analysis, photopic blink reflex response (PBR), electroretinography (ERG) and visual behavior testing before and after treatment with IVIg and steroid therapy. Optical coherence tomography (OCT) was performed to evaluate retinal thickness.

Results: : Molecular analysis of SARDS retinas showed extensive intra-retinal presence of immunoglobulin rich plasma cells in all stages of disease. Microarray analysis of SARDS retinal tissue showed significant up-regulation of genes responsible for immunoglobulin synthesis (6-147 fold increase). Complement genes were significantly up-regulated in SARDS eyes (3-10 fold increase), which was also confirmed by immunohistochemistry. Optical coherence tomography showed significant thinning of the nerve fiber layer and total retinal thickness of the inferior retina in SARDS patients. All SARDS patients improved visual function and photoreceptor-mediated PLR responses after treatment.

Conclusions: : SARDS is a spontaneously occurring canine model of antibody-mediated retinopathy with a novel mechanism of antibody production from intra-retinal infiltrated plasma cells. This syndrome is at least partially responsive to the IVIg therapy.

Keywords: retinitis • electroretinography: clinical • gene microarray 
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