May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Characterization of Retinal Pigment Epithelium Cells Isolated From Cynomolgus Monkey With Early-Onset Macular Degeneration
Author Affiliations & Notes
  • Z.-L. Chi
    Nt'l Inst of Sensory Organs,, Nt'l Tokyo Medical Center,, Tokyo, Japan
  • H. Okamoto
    Nt'l Inst of Sensory Organs,, Nt'l Tokyo Medical Center,, Tokyo, Japan
  • M. Suzuki
    The Corporation for Production and Research of Laboratory Primates, Tokyo, Japan
  • K. Terao
    Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Tokyo, Japan
  • Y. Yoshikawa
    Graduate School of Agricultual and Life Sciences, The University of Tokyo, Tokyo, Japan
  • T. Iwata
    Nt'l Inst of Sensory Organs,, Nt'l Tokyo Medical Center,, Tokyo, Japan
  • Footnotes
    Commercial Relationships  Z. Chi, None; H. Okamoto, None; M. Suzuki, None; K. Terao, None; Y. Yoshikawa, None; T. Iwata, None.
  • Footnotes
    Support  Ministry of Health, Labour and Welfare of Japan
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 215. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Z.-L. Chi, H. Okamoto, M. Suzuki, K. Terao, Y. Yoshikawa, T. Iwata; Characterization of Retinal Pigment Epithelium Cells Isolated From Cynomolgus Monkey With Early-Onset Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 2008;49(13):215. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : We have previously reported a cynomolgus (Macaca fascicularis) pedigree with early-onset macular degeneration. These monkeys show cardinal features of age-related macular degeneration in human such as pigmentary disorders and/or drusen-like spots at two years after birth (Umeda et al., 2005) and ERG abnormality. Here we report a characterization of retinal pigment epithelium (RPE) cells isolated from two affected monkeys.

Methods: : RPE cells were isolated from wild type (11 and 14 yrs old) and affected monkeys with severe (17 yrs old) and modest (19 yrs old) disease progression based on fundus photograph, ERG, and fluorescence fundus photograph. The RPE cells were used for cell marker staining, proliferation assay, phagocytosis analysis (Olympus, LCV100), gene expression analysis using cDNA microarray, proteome analysis by 2D-gel electrophoresis and LC-MS/MS mass spectrometer (ThermoElectron, LCQ DECA XP Plus).

Results: : RPE cells from affected monkeys were significantly reduced of proliferation speed and phagocytosis activity. These RPE cells were also unable to form tight junction observed by Zonula Occludens-1 (ZO-1) immunostaining. Genes and proteins expression of RPE cells were significantly exhibiting significant expression changed at different disease stages compared with normal RPE cells.

Conclusions: : In this study, primary RPE cells from affected and control monkeys were analyzed. Significant change of gene and protein expression leading to loss of tight junction in RPE cells suggests that barrier function between neural retina and choroid maybe the cause of the disease.

Keywords: age-related macular degeneration • retinal pigment epithelium • proteomics 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×