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H. Kim, J. Kim, J. Kim, Y. Yu, D. Kim, K.-W. Kim; Arsenic Trioxide Induces the Differentiation or Apoptosis of Retinoblastoma Cells Through MAPK Pathways. Invest. Ophthalmol. Vis. Sci. 2008;49(13):27. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To assess the effect of arsenic trioxide (As2O3) on the differentiation or apoptosis of retinoblastoma cells depending on its concentration
Y79 and SNUOT-Rb1 retinoblastoma cells were treated with As2O3 in a concentration dependent manner (0.1 ~ 10 υM). Fluorocytometry and DAPI staining were performed to confirm the differentiation or apoptosis in retinoblastoma cells. To investigate the relationship between differentiation or apoptosis and mitogen activated protein kinase pathways, western blotting for ERK-1/2 & c-JNK was performed.
As2O3 at low concentrations (0.1-1 υM) induced differentiation of retinoblastoma cells, when ERK2 was activated and PD98059, an inhibitor of MEK-1, blocked the differentiation by As2O3. High concentrations (2-10 υM) of As2O3 induced the apoptosis which was accompanied by the activation of c-JNK.
Our data suggests that As2O3 has a strong therapeutic potential for the treatment of retinoblastoma cells.
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