May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Arsenic Trioxide Induces the Differentiation or Apoptosis of Retinoblastoma Cells Through MAPK Pathways
Author Affiliations & Notes
  • H. Kim
    Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seongnam-si, Republic of Korea
    Thoracic and cardiovascular surgery, Seoul National University Bundang hospital, Seongnam-si, Republic of Korea
  • J. Kim
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • J. Kim
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Y. Yu
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • D. Kim
    Radiology, Chosun University, Gwang Ju, Republic of Korea
  • K.-W. Kim
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  H. Kim, None; J. Kim, None; J. Kim, None; Y. Yu, None; D. Kim, None; K. Kim, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 27. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      H. Kim, J. Kim, J. Kim, Y. Yu, D. Kim, K.-W. Kim; Arsenic Trioxide Induces the Differentiation or Apoptosis of Retinoblastoma Cells Through MAPK Pathways. Invest. Ophthalmol. Vis. Sci. 2008;49(13):27.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To assess the effect of arsenic trioxide (As2O3) on the differentiation or apoptosis of retinoblastoma cells depending on its concentration

Methods: : Y79 and SNUOT-Rb1 retinoblastoma cells were treated with As2O3 in a concentration dependent manner (0.1 ~ 10 υM). Fluorocytometry and DAPI staining were performed to confirm the differentiation or apoptosis in retinoblastoma cells. To investigate the relationship between differentiation or apoptosis and mitogen activated protein kinase pathways, western blotting for ERK-1/2 & c-JNK was performed.

Results: : As2O3 at low concentrations (0.1-1 υM) induced differentiation of retinoblastoma cells, when ERK2 was activated and PD98059, an inhibitor of MEK-1, blocked the differentiation by As2O3. High concentrations (2-10 υM) of As2O3 induced the apoptosis which was accompanied by the activation of c-JNK.

Conclusions: : Our data suggests that As2O3 has a strong therapeutic potential for the treatment of retinoblastoma cells.

Keywords: retinoblastoma • differentiation • apoptosis/cell death 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×