May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Ovariectomy Persistently Increases the Gene Expression and Protein Levels of Il-1β, Ifn- and Cxcl13 Over Time
Author Affiliations & Notes
  • V. Seamon
    Charles E. Schmidt College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida
  • H. Quinonez
    Charles E. Schmidt College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida
  • A. Azzarolo
    Charles E. Schmidt College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida
  • Footnotes
    Commercial Relationships  V. Seamon, None; H. Quinonez, None; A. Azzarolo, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 429. doi:https://doi.org/
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      V. Seamon, H. Quinonez, A. Azzarolo; Ovariectomy Persistently Increases the Gene Expression and Protein Levels of Il-1β, Ifn- and Cxcl13 Over Time. Invest. Ophthalmol. Vis. Sci. 2008;49(13):429. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Sjogren’s syndrome (SS), characterized by lacrimal dysfunction and destruction as well as lymphocytic infiltration, primarily affects post-menopausal women whose levels of sex hormones are significantly decreased. However, not all post-menopausal women develop SS. Therefore, we hypothesized that a decline in the circulating levels of sex hormones in a genetically predisposed murine model of SS, will cause an increase in the gene expression and protein levels of IL-1β, IFN-γ and CXCL13, in the lacrimal glands, which becomes more severe and persistent with time compared to a non-genetically predisposed murine strain.

Methods: : Six wks old C57BL/10SnJ, control, and NOD.B10-H2b, mouse model of SS, were ovariectomized (OVX) or sham operated. After 3, 7 or 21 days, the lacrimal glands were removed, pooled, and homogenized. Total RNA was used to analyze gene expression levels of IL-1β, IFN-γ and CXCL13. ELISA analysis was used to determine protein concentration of IL-1β, IFN-γ and CXCL13.

Results: : Ovariectomized NOD mice showed significant and persistent increases in the gene expression and protein levels of IL-1β, IFN-γ and CXCL13 compared to sham operated animals. No significant changes were seen in the gene expression or protein levels of IL-1β, IFN-γ and CXCL13 between the sham and OVX in C57BL/10SnJ mice at any of the experimental time points.

Conclusions: : These results support our hypothesis that reduction of the circulatory levels of sex hormones causes an increase in the expression of IL-1β, IFN-γ and CXCL13 in a genetically predisposed murine model of SS. Since this increase becomes more severe and persistent with time, it is reasonable to assume that sex hormones in addition to a genetic predisposition are necessary factors in the development of SS.

Keywords: lacrimal gland • cytokines/chemokines 
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