May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Topical Cyclosporine Emulsion Modulates Immune Response in Experimental Dry Eye
Author Affiliations & Notes
  • S. B. Pangelinan
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • C. S. De Paiva
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • R. Singh
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • K. Agusala
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • W. J. Farley
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • M. Stern
    Ophthalmology, Allergan Inc., Irvine, California
  • S. C. Pflugfelder
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • Footnotes
    Commercial Relationships  S.B. Pangelinan, None; C.S. De Paiva, None; R. Singh, None; K. Agusala, None; W.J. Farley, None; M. Stern, Allergan Inc., E; S.C. Pflugfelder, None.
  • Footnotes
    Support  NIH Grant EY 11915 (SCP), an unrestricted grant from Research to Prevent Blindness, The Oshman Foundation, The William Stamps Farish Fund, The Hamill Foundation and an unrestricted grant from Allergan
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 440. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      S. B. Pangelinan, C. S. De Paiva, R. Singh, K. Agusala, W. J. Farley, M. Stern, S. C. Pflugfelder; Topical Cyclosporine Emulsion Modulates Immune Response in Experimental Dry Eye. Invest. Ophthalmol. Vis. Sci. 2008;49(13):440. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To investigate the effect of topical Cyclosporine (CsA) 0.05% treatment on the local immune response to experimental murine dry eye.

Methods: : Experimental dry eye (EDE) was induced in 3 groups of C57BL6 mice by cholinergic blockade and exposure to a desiccating environment for 5 days. The dry eye control group received no topical therapy, a second group received 1uL of 0.05% cyclosporine ophthalmic emulsion QID (EDE + CsA) and a third group received 1uL of CsA vehicle (polysorbate 80 1%, glycerin 1%) QID. Eyes and ocular adnexa were excised, cryosectioned, and evaluated for goblet cell density, CD4+ and CD8+ T cell infiltration and TGFβ and CD103 expression. Goblet cells were counted in periodic acid Shiff (PAS)-stained sections. Antigens were detected by immunohistochemistry and immunofluorescent confocal microscopy.

Results: : Dry eye induced migration of CD4+ T cells into the goblet cell rich area of the conjunctival epithelium, the density of CD8+ T cells; CD103 and TGFβ2 staining decreased in the same area. Treatment with topical CsA reduced CD4+ T cell migration and promoted a significant increase in CD8+ T cells in comparison to the EDE 5D control group. Goblet cell density was maintained in the CsA group. A significant direct correlation was noted between the density of CD8+ T cells and goblet cells (p<0.001). The CsA treated group had a significantly greater number of CD103+ cells and greater TGFβ2 expression than the EDE 5D or the vehicle groups.

Conclusions: : Topical CsA significantly reduces conjunctival CD4+ T cell infiltration, and promotes intraepithelial CD8+ T cell migration protecting against the goblet cell loss in experimental murine dry eye.

Keywords: cornea: tears/tear film/dry eye • immunomodulation/immunoregulation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×