May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Molecular Regulation of Uveal Melanoma Cell Vaculogenic Mimicry by Helix-Loop-Helix Id2 (Inhibitor of DNA Binding)
Author Affiliations & Notes
  • B. Li
    Beijing Tongren Hospital, Beijing, China
    Lab of Ophthalmic Pathology,
  • F. Su
    Beijing Tongren Hospital, Beijing, China
    Lab of Ophthalmic Pathology,
  • J. Wang
    Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing, China
  • L. Q. Li
    Beijing Tongren Hospital, Beijing, China
    Lab of Ophthalmic Pathology,
  • F. Gao
    Beijing Tongren Hospital, Beijing, China
    Lab of Ophthalmic Pathology,
  • X. L. Xu
    Beijing Tongren Hospital, Beijing, China
    Lab of Ophthalmic Pathology,
  • X. C. Liu
    Beijing Tongren Hospital, Beijing, China
    Central Laboratory,
  • Footnotes
    Commercial Relationships  B. Li, None; F. Su, None; J. Wang, None; L.Q. Li, None; F. Gao, None; X.L. Xu, None; X.C. Liu, None.
  • Footnotes
    Support  Beijing Municipal Natural Science Foundation(7053065)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 50. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      B. Li, F. Su, J. Wang, L. Q. Li, F. Gao, X. L. Xu, X. C. Liu; Molecular Regulation of Uveal Melanoma Cell Vaculogenic Mimicry by Helix-Loop-Helix Id2 (Inhibitor of DNA Binding). Invest. Ophthalmol. Vis. Sci. 2008;49(13):50. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To examine whether the helix-loop-helix Id2 (inhibitor of DNA binding) is involved in the process of human uveal melanoma vasculogenic mimicry.

Methods: : Paraffin-embedded tissue sections from primary uveal melanoma patients were underwent immunohistochemistry staining for testing Id-2 expression in human melanomas, and PAS staining was used to determine the presence of vasculogenic mimicry. The highly and poorly aggressive human uveal melanoma cells were treated with Id-2 RNA interference when cultured on a three dimensional matrix.

Results: : Id-2 expression was found decreased in human uveal melanoma tissues versus normal uvea tissues. In immunohistochemistry analysis, tumors with low Id-2 expression which was graded scores 2 and 3 significantly had more chances to form vasculogenic mimicry than tumors with high Id-2 expression which was graded scores 4 and 5 ( P< 0.01 ). In three-dimension cultures in vitro the highly aggressive cells can form tubular networks wherease the poorly aggressive cells can not form the tubular networks. And the observation demonstrated that highly aggressive melanoma cells were abrogated the capability of forming vasvulogenic mimicry whentransient knocked down of Id-2.

Conclusions: : These results suggest that Id-2 was involved in forming tubular networks in highly aggressive melanoma cells in vitro, which may represent a novel therapeutic target for further clinical investigation.

Keywords: melanoma • uvea • tumors 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×