Purchase this article with an account.
F. F. Behar-Cohen, L. Kowalczuk, E. Touchard, P. Bigey, D. Scherman, N. Miyamoto; Intraocular Over Expression of Placental Growth Factor-1 (PlGF-1) Induces Corneal But No Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2008;49(13):515. doi: https://doi.org/.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
1-2 months (rdy+-p) rats were used (6 eyes per conditions). Rat PlGF-1 was cloned in pVAX2 backbone plasmid. Injections of 10µl (30µg) of pVAX1-LacZ or pVAX2-PlGF-1 plasmids (driven by a CMV promoter) in PBS were performed either into the ciliary muscle or the subretinal spaces of rat eyes. Control eyes received ET of PBS. An iridium/platinum needle electrode was placed at the injection sites and a return plaque electrode was placed on the sclera. Electrical parameters were chosen to reach a 200V/cm field, with 8 pulses 20 ms duration (5Hz). Transfection was evaluated at one week using macroscopic examination for b-gal activity on histological sections. Clinical examination was performed at day 2, 8, 15, 30, 100 and semi-thin sections were performed at 30 and 100 days.
ET allowed for a safe an efficient transfection of ciliary muscle cells, or the retinal pigment epithelial cells (RPE) and for PlGF-1 secretion. At day 2, eyes that had received ET of PlGF-1 did not show any difference when compared control eyes. At day 8 and 30, when ET was performed in the ciliary muscle, intense central corneal neo vascularizations associated to vasodilatation was observed without any retinal neovascularization. When PlGF-1 was over expressed in RPE cells, no neovascularization was observed in the retina but on histology, sub retinal fluid accumulation along with retinal edema was observed.
This PDF is available to Subscribers Only