May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Intraocular Over Expression of Placental Growth Factor-1 (PlGF-1) Induces Corneal But No Choroidal Neovascularization
Author Affiliations & Notes
  • F. F. Behar-Cohen
    Team 17, Physiopathology of Ocular Diseases: Therapeutic Innovations, INSERM UMR S 872, Les Cordeliers, Université Pierre et Marie Curie, Université Paris Descartes, Paris, France
    Ophthalmology, Hotel-Dieu of Paris, Paris Descartes University, Paris, France
  • L. Kowalczuk
    Team 17, Physiopathology of Ocular Diseases: Therapeutic Innovations, INSERM UMR S 872, Les Cordeliers, Université Pierre et Marie Curie, Université Paris Descartes, Paris, France
  • E. Touchard
    Team 17, Physiopathology of Ocular Diseases: Therapeutic Innovations, INSERM UMR S 872, Les Cordeliers, Université Pierre et Marie Curie, Université Paris Descartes, Paris, France
  • P. Bigey
    INSERM U640, Paris, France
  • D. Scherman
    INSERM U640, Paris, France
  • N. Miyamoto
    Team 17, Physiopathology of Ocular Diseases: Therapeutic Innovations, INSERM UMR S 872, Les Cordeliers, Université Pierre et Marie Curie, Université Paris Descartes, Paris, France
  • Footnotes
    Commercial Relationships  F.F. Behar-Cohen, None; L. Kowalczuk, None; E. Touchard, None; P. Bigey, None; D. Scherman, None; N. Miyamoto, None.
  • Footnotes
    Support  Work supported by the European project EVI-GENORET (LSHG-CT-2005-512036) and the ANR EMERGENCE project (ANR05EMPB001-02).
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 515. doi:https://doi.org/
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    • Get Citation

      F. F. Behar-Cohen, L. Kowalczuk, E. Touchard, P. Bigey, D. Scherman, N. Miyamoto; Intraocular Over Expression of Placental Growth Factor-1 (PlGF-1) Induces Corneal But No Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2008;49(13):515. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Methods: : 1-2 months (rdy+-p) rats were used (6 eyes per conditions). Rat PlGF-1 was cloned in pVAX2 backbone plasmid. Injections of 10µl (30µg) of pVAX1-LacZ or pVAX2-PlGF-1 plasmids (driven by a CMV promoter) in PBS were performed either into the ciliary muscle or the subretinal spaces of rat eyes. Control eyes received ET of PBS. An iridium/platinum needle electrode was placed at the injection sites and a return plaque electrode was placed on the sclera. Electrical parameters were chosen to reach a 200V/cm field, with 8 pulses 20 ms duration (5Hz). Transfection was evaluated at one week using macroscopic examination for b-gal activity on histological sections. Clinical examination was performed at day 2, 8, 15, 30, 100 and semi-thin sections were performed at 30 and 100 days.

Results: : ET allowed for a safe an efficient transfection of ciliary muscle cells, or the retinal pigment epithelial cells (RPE) and for PlGF-1 secretion. At day 2, eyes that had received ET of PlGF-1 did not show any difference when compared control eyes. At day 8 and 30, when ET was performed in the ciliary muscle, intense central corneal neo vascularizations associated to vasodilatation was observed without any retinal neovascularization. When PlGF-1 was over expressed in RPE cells, no neovascularization was observed in the retina but on histology, sub retinal fluid accumulation along with retinal edema was observed.

Keywords: growth factors/growth factor receptors • gene transfer/gene therapy • neovascularization 
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