May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Correlation of Chromosome 3 Monosomy and Histopathologic Features in Choroidal Melanoma
Author Affiliations & Notes
  • J. Ulrich
    Ophthalmology, Geisinger Medical Center, Danville, Pennsylvania
  • M. A. Materin
    Ocular Oncology,
    Wills Eye Institute, Philadelphia, Pennsylvania
  • C. L. Shields
    Ocular Oncology,
    Wills Eye Institute, Philadelphia, Pennsylvania
  • R. C. Eagle, Jr.
    Ocular Pathology,
    Wills Eye Institute, Philadelphia, Pennsylvania
  • Footnotes
    Commercial Relationships  J. Ulrich, None; M.A. Materin, None; C.L. Shields, None; R.C. Eagle, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 52. doi:https://doi.org/
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      J. Ulrich, M. A. Materin, C. L. Shields, R. C. Eagle, Jr.; Correlation of Chromosome 3 Monosomy and Histopathologic Features in Choroidal Melanoma. Invest. Ophthalmol. Vis. Sci. 2008;49(13):52. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To compare histopathology and chromosome 3 mutations of choroidal melanomas in patients undergoing enucleation.

Methods: : 40 choroidal melanomas of 40 consecutive patients undergoing enucleation were evaluated for chromosome 3 monosomy by fluorescence in situ hybridization (FISH). Histopathology studies were performed grading the specimens in regards to basal diameter, mitotic activity, presence of lymphocytes and melanoma cytology. A total score was calculated and chromosome 3 monosomy patients were compared to chromosome 3 disomy patients.

Results: : FISH revealed chromosome 3 monosomy in 25 patients and disomy in 15 patients. Patients with monosomy 3 had a statistically significant higher lymphocyte score (1.32 vs. 1.0; p = 0.0151) and total histopathology score (11.1 vs. 9.3; p = 0.0085) than disomy 3 patients. The remaining individual criteria showed a similar trend but were not statistically significant.

Conclusions: : In patients with choroidal melanoma requiring enucleation, chromosome 3 monosomy, which is a poor prognostic factor, seems to be correlated with an increased amount of atypia on histopathology.

Keywords: melanoma • microscopy: light/fluorescence/immunohistochemistry • genetics 
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