May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Inhibition of Choroidal Neovascularization With an Anti-Inflammatory Carotenoid Astaxanthin
Author Affiliations & Notes
  • K. Izumi-Nagai
    Keio University School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Department of Ophthalmology,
  • N. Nagai
    Keio University School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Department of Ophthalmology,
  • K. Ohgami
    Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • S. Satofuka
    Keio University School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Department of Ophthalmology,
  • Y. Ozawa
    Keio University School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Department of Ophthalmology,
  • K. Tsubota
    Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology,
  • S. Ohno
    Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Y. Oike
    Keio University School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Department of Molecular Genetics, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan
  • S. Ishida
    Keio University School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Department of Ophthalmology,
  • Footnotes
    Commercial Relationships  K. Izumi-Nagai, None; N. Nagai, None; K. Ohgami, None; S. Satofuka, None; Y. Ozawa, None; K. Tsubota, None; S. Ohno, None; Y. Oike, None; S. Ishida, None.
  • Footnotes
    Support  Grant-in-aid for Scientific Research of Japanese Ministry of Education, Culture, Sports, Science and Technology (No.19592039 to S.I.)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 533. doi:https://doi.org/
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    • Get Citation

      K. Izumi-Nagai, N. Nagai, K. Ohgami, S. Satofuka, Y. Ozawa, K. Tsubota, S. Ohno, Y. Oike, S. Ishida; Inhibition of Choroidal Neovascularization With an Anti-Inflammatory Carotenoid Astaxanthin. Invest. Ophthalmol. Vis. Sci. 2008;49(13):533. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Astaxanthin (AST) is a carotenoid found in marine animals and vegetables. The aim of the present study was to investigate the effect of AST on the development of experimental choroidal neovascularization (CNV) with underlying cellular and molecular mechanisms.

Methods: : Laser photocoagulation was used to induce CNV in C57BL/6J mice. Mice were pretreated with an intraperitoneal injection of AST daily for 3 days before photocoagulation and the treatment were continued daily till the end of the study. CNV response was analyzed by volumetric measurements 1 week after laser injury. Retinal pigment epithelium (RPE)-choroidal levels of IΚB-α, intercellular adhesion molecule (ICAM)-1, monocyte chemotactic protein (MCP)-1, interleukin (IL)-6, vascular endothelial growth factor (VEGF), VEGF receptor-1 (VEGFR-1) and VEGFR-2 were examined by western blotting or ELISA. AST was applied to capillary endothelial (b-End3) cells, macrophages and RPE cells to analyze the activation of NF-ΚB and the expression of inflammatory molecules.

Results: : The index of CNV volume was significantly suppressed by the treatment with AST, compared with vehicle-treated animals. AST treatment led to significant inhibition of macrophage infiltration into CNV and of the in vivo and in vitro expression of inflammation-related molecules including VEGF, IL-6, ICAM-1, MCP-1, VEGFR-1 and VEGFR-2. Importantly, AST suppressed the activation of the NF-ΚB pathway including IΚB-α degradation and p65 nuclear translocation.

Conclusions: : AST treatment led to significant suppression of CNV development together with inflammatory processes including NF-ΚB activation, subsequent upregulation of inflammatory molecules and macrophage infiltration. The present study suggests the possibility of AST supplementation as a therapeutic strategy to suppress CNV associated with AMD.

Keywords: age-related macular degeneration • choroid: neovascularization • carotenoids/carotenoid binding proteins 
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