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Y. Liu, M. X. Zhang, D. Lan, J. J. Zhang; Expression of Tissue Factor in Choroidal Melanoma and Its Clinical Significance. Invest. Ophthalmol. Vis. Sci. 2008;49(13):58.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the expression of tissue factor (TF) in choroidal melanoma (CM) cell line and in human choroidal melanoma, and to study its specificity that can provide some evidence on therapy of CM.
CM cells (OCM-1) were cultured in vitro and identified by observing the morphous.TF expression was studied with immunohistochemical staining method in OCM-1, and in specimens with 10 cases of CM and 5 cases of human normal choroidal tissue, with its clinic significance analyzed.
OCM-1 growth curve was similar to "S" type, which included the adaptive phase in first 12h, then entered into logarithmic growth phase, and finally into platform phase by 3d. TF protein was mainly expressed in cytoplasm of OCM-1 (positive cell percentage 85.33%±5.47%). Among 10 human choroidal melanoma tissue, TF protein was mainly expressed in cytoplasm of CM cell cytoplasm (positive cell percentage 41.60%±14.17%, IOD 33853.67±16445.30). Among 5 human normal choroidal tissue, TF protein was negative expressed.
The result suggested TF peculiarly expressed in both CM cell line and human choroidal melanoma tissue may play a role as an immunotherapy target of CM, and provided some evidence on target-therapy of CM.
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