May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
SPARC Expression Correlates with Proliferative and Invasive Potential in Uveal Melanoma Cell Lines
Author Affiliations & Notes
  • S. Di Cesare
    Ocular Pathology, McGill University, Montreal, Quebec, Canada
  • S. Maloney
    Ocular Pathology, McGill University, Montreal, Quebec, Canada
  • R. N. Belfort
    Ocular Pathology, McGill University, Montreal, Quebec, Canada
  • B. F. Fernandes
    Ocular Pathology, McGill University, Montreal, Quebec, Canada
  • P. Logan
    Ocular Pathology, McGill University, Montreal, Quebec, Canada
  • M. N. Burnier, Jr.
    Ocular Pathology, McGill University, Montreal, Quebec, Canada
  • Footnotes
    Commercial Relationships  S. Di Cesare, None; S. Maloney, None; R.N. Belfort, None; B.F. Fernandes, None; P. Logan, None; M.N. Burnier, None.
  • Footnotes
    Support  Cedars Cancer Institue
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 61. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      S. Di Cesare, S. Maloney, R. N. Belfort, B. F. Fernandes, P. Logan, M. N. Burnier, Jr.; SPARC Expression Correlates with Proliferative and Invasive Potential in Uveal Melanoma Cell Lines. Invest. Ophthalmol. Vis. Sci. 2008;49(13):61. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Secreted protein acidic and rich in cisteine (SPARC) has been shown to play an integral role in the progression of numerous malignancies. It is associated with general tissue remodeling and angiogenesis. SPARC expression has been correlated with good prognosis in some cancers and poor prognosis in others. The aim of this study was to determine expression of SPARC in four uveal melanoma cell lines, and one transformed melanocyte cell line. Furthermore, we sought to investigate its role on proliferation and invasion in vitro.

Methods: : Immunocytochemistry was performed on cytospins of the five cell lines using a monoclonal mouse anti-human antibody to SPARC. Quantitative real-time PCR (Chromo4 thermocycler) was used to demonstrate mRNA levels of SPARC expression in four human uveal melanoma cell lines (92.1, SP6.5, MKT-BR, OCM-1) and one transormed melanocyte cell line (UW-1). SPARC HP genome wide designed siRNA was used (Qiagen) to transfect each of the five cell lines. Flourescently labeled non-silencing control siRNA (Qiagen) was used for detection of transfection efficiency as well used as an appropriate control. Transfected cells were subsequently analyzed using proliferation and invasion assays and compared to appropriate controls.

Results: : Expression of SPARC at the RNA level was demonstrated using quantitative real-time PCR and confirmed at the protein level by immunocytochemistry for all five cell lines. The cell lines transfected with siRNA directed against SPARC showed a significant decrease in proliferation (p<0.01) when compared to the control groups. Transfected cell lines similarly showed a significant decrease (p<0.01) in invasive ability.

Conclusions: : SPARC expression appears to be associated with proliferative and invasive capacity of human uveal melanoma cell lines. Targeting this matricellular protein may be one approach to manage malignant progression of uveal melanoma in affected patients.

Keywords: uvea • pathobiology • oncology 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×