May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Global Hyperdeimination and Local Hypodeimination in Multiple Sclerosis Retinas
Author Affiliations & Notes
  • S. K. Bhattacharya
    Bascom Palmer Eye Institute, University of Miami Miller Sch of Med, Miami, Florida
  • M. E. Algeciras
    Bascom Palmer Eye Institute, University of Miami Miller Sch of Med, Miami, Florida
  • R. Marc
    Moran Eye Center, University of Utah, Salt Lake City, Utah
  • F. G. Mastronardi
    Dept. Molecular Structure & Function, The Hospital for Sick Children, Toronto, Ontario, Canada
  • Footnotes
    Commercial Relationships  S.K. Bhattacharya, None; M.E. Algeciras, None; R. Marc, None; F.G. Mastronardi, None.
  • Footnotes
    Support  AHAF (SKB), RPB Career Award (SKB), NIH Center Grant P30EY14801; an unrestricted grant from Research to Prevent Blindness.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 767. doi:https://doi.org/
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    • Get Citation

      S. K. Bhattacharya, M. E. Algeciras, R. Marc, F. G. Mastronardi; Global Hyperdeimination and Local Hypodeimination in Multiple Sclerosis Retinas. Invest. Ophthalmol. Vis. Sci. 2008;49(13):767. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The purpose of this investigation is to gain insight into the role of Peptidyl arginine deiminase 2 (PAD2) and protein deimination in multiple sclerosis (MS) retinas. Elevated PAD2, enzyme, that converts protein-bound arginine to citrulline (deimination) have been previously reported in MS.

Methods: : Western and immunohistochemical analyses measured PAD2 expression and protein deimination in MS (ND4 mouse) and control mouse retina (10 animals each) and human retinas (10 each control and MS). Select proteins from retinal ganglion cells of control eyes were identified by liquid chromatography tandem mass spectrometry following immunoprecipitation using antibodies that bind to deiminated proteins. The studies were performed adhering to declaration of tenets of Helsinki and ARVO statement for animal use in Ophthalmic and visual research.

Results: : Elevated PAD2 and protein deimination was found in MS mouse retina in contrast to controls by immunohistochemistry and were also confirmed by Western analysis. However, closer examination of MS eyes (mouse and human) in contrast to controls showed decreased protein deimination in retinal ganglion cell layer initially by immunohistochemistry and was confirmed by Western analysis.

Conclusions: : Our results revealed the presence of local hypodeimination in MS retinal ganglion cells in addition to global hyperdeimination in the retina. We have further identified two proteins that undergo loss of deimination in MS.

Keywords: protein modifications-post translational • inner retina dysfunction: biochemistry and cell biology • retina 
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