May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Quantitative Presence of the Ca2+ Sensor S100B-Modulated ROS-GC1 Transduction Machinery in the Retinal Neurons Compartments
Author Affiliations & Notes
  • T. M. Duda
    Regulatory & Molecular Biology, Pennsylvania College of Optometry, Elkins Park, Pennsylvania
  • V. Venkataraman
    Cell Biology, UMDNJ-SOM, Stratford, New Jersey
  • R. K. Sharma
    Regulatory & Molecular Biology, Pennsylvania College of Optometry, Elkins Park, Pennsylvania
  • Footnotes
    Commercial Relationships  T.M. Duda, None; V. Venkataraman, None; R.K. Sharma, None.
  • Footnotes
    Support  NIH awards DC 005349 (RKS), HL 084584 (TD), UMDNJ and Lindback Foundations (VV)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 770. doi:https://doi.org/
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      T. M. Duda, V. Venkataraman, R. K. Sharma; Quantitative Presence of the Ca2+ Sensor S100B-Modulated ROS-GC1 Transduction Machinery in the Retinal Neurons Compartments. Invest. Ophthalmol. Vis. Sci. 2008;49(13):770. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To make a quantitative assessment on the functional presence of the S100B-modulated ROS-GC1 transduction system in the outer segments of photoreceptors, outer (P1) and inner (P2) plexiform layers of the mouse retina.

Methods: : Biochemical techniques involving guanylate cyclase assays, Western blots and co-immunoprecipitation were used on the membranes of the genetically modified mouse model systems consisting of S100B gene knock-out (k/o), ROS-GC1 k/o, and GCAP1-Y99C.

Results: : There are four membrane guanylate cyclases in the retina. Two are surface receptors, ANF-RGC and CNP-RGC; and two are Ca2+-modulated, ROS-GC1 and ROS-GC2. The ROS-GC1 percentage presence in the following native compartments of the retina is as follows: outer segments, 95%; P1, 60%; and P2, 50%. The contribution of the S100B-modulation in these native systems is as follows: outer segments, 45%; P1, 30%; and P2, 25%.

Conclusions: : 1) The major phototransduction linked membrane guanylate cyclase is ROS-GC1. ROS-GC2 (4%) has a minor yet significant role. 2) Ca2+-bound S100B modulates the native ROS-GC1 in the three retinal layers--outer segments, P1 and P2. 3) Peptide hormone receptor guanylate cyclases, ANF-RGC and CNP-RGC, have also significant presence in all three retinal layers.

Keywords: calcium • signal transduction • retina: distal (photoreceptors, horizontal cells, bipolar cells) 
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