May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Microarray Identification of Transcripts Enriched in Zebrafish Cones
Author Affiliations & Notes
  • A. Glaviano
    Conway Institute, University College Dublin, Dublin, Ireland
  • S. McLoughlin
    Conway Institute, University College Dublin, Dublin, Ireland
  • Y. Alvarez
    Conway Institute, University College Dublin, Dublin, Ireland
  • A. Blanco
    Conway Institute, University College Dublin, Dublin, Ireland
  • B. Sapetto-Rebow
    Conway Institute, University College Dublin, Dublin, Ireland
  • P. Ó Gaora
    Conway Institute, University College Dublin, Dublin, Ireland
  • B. Kennedy
    Conway Institute, University College Dublin, Dublin, Ireland
  • Footnotes
    Commercial Relationships  A. Glaviano, None; S. McLoughlin, None; Y. Alvarez, None; A. Blanco, None; B. Sapetto-Rebow, None; P. Ó Gaora, None; B. Kennedy, None.
  • Footnotes
    Support  Science Foundation Ireland Investigator Programme Grant 04/IN3/B559 (BNK); Macular Degeneration Research, a programme of the Americal Health Assistance Foundation (BNK)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 781. doi:https://doi.org/
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    • Get Citation

      A. Glaviano, S. McLoughlin, Y. Alvarez, A. Blanco, B. Sapetto-Rebow, P. Ó Gaora, B. Kennedy; Microarray Identification of Transcripts Enriched in Zebrafish Cones. Invest. Ophthalmol. Vis. Sci. 2008;49(13):781. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : . Human blindness can result from functional loss of rod and/or cone photoreceptors. Loss of cone photoreceptors results in severe visual handicap in rare genetic diseases e.g. achromatopsia and in prevalent complex diseases e.g. age-related macular degeneration. Therefore, we sought to identify genes enriched in cone photoreceptors that represent therapeutic targets to slow the severity of blindness.

Methods: : . A transgeniczebrafish line expressing green fluorescent protein (EGFP) in cone photoreceptors was used (Kennedy et al 2007, IOVS 48: 522-529). Retinas from adult fish were dissected, dissociated and sorted by FACS into EGFP+ (cone photoreceptors) and EGFP- cells. High quality RNA was isolated and microarray analysis performed.

Results: : . RT-PCR confirmed that cone photoreceptors were highly enriched by FACS purification. Among the 15,617 probesets on the chip, 1,838 show statistically differential expression in cone photoreceptors versus the remaining non-EGFP retinal cells. Several gene ontology terms were found to be significantly overrepresented in the set of differentially expressed genes.

Conclusions: : . Gene ontology and pathway mapping analysis will be used to select genes for functional studies. In-situ hybridization and morpholino knockdown approaches are currently been applied to identify gene subsets that are essential for cone photoreceptor function and integrity.

Keywords: gene microarray • genetics • gene/expression 
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