May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Protein Profile Comparison of Primary Human Uveal Melanoma With Disomy versus Monosomy of Chromosome 3
Author Affiliations & Notes
  • H. Vorum
    Aarhus University Hospital, Aarhus, Denmark
    Department of Ophthalmology,
    Institute of Medical Biochemistry, University of Aarhus, Aarhus, Denmark
  • S. Coupland
    Department of Pathology, University of Liverpool, Liverpool, United Kingdom
  • N. Mandal
    Aarhus University Hospital, Aarhus, Denmark
    Institute of Medical Biochemistry,
  • B. Honoré
    Institute of Medical Biochemistry, University of Aarhus, Aarhus, Denmark
  • S. Urbak
    Aarhus University Hospital, Aarhus, Denmark
    Department of Ophthalmology,
  • B. Damato
    St. Paul's Eye Unit, Royal Liverpool University Hospital, Liverpool, United Kingdom
  • Footnotes
    Commercial Relationships  H. Vorum, None; S. Coupland, None; N. Mandal, None; B. Honoré, None; S. Urbak, None; B. Damato, None.
  • Footnotes
    Support  Danish Medical Research Council
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 790. doi:https://doi.org/
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      H. Vorum, S. Coupland, N. Mandal, B. Honoré, S. Urbak, B. Damato; Protein Profile Comparison of Primary Human Uveal Melanoma With Disomy versus Monosomy of Chromosome 3. Invest. Ophthalmol. Vis. Sci. 2008;49(13):790. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To compare the proteomic profiles of primary uveal melanoma with and without loss of chromosome 3.

Methods: : Three tumors with disomy 3 and four tumors with monosomy 3 were subjected to high resolution two-dimensional gel electrophoresis. Following development of individual protein spots by silver staining, the expression profile of the pattern of proteins was identified. Spots that differed between the two groups were excised and analysed by tandem mass spectrometry. Further analysis was undertaken with immunoblotting to confirm findings.

Results: : The overall protein expression profiles of the tumors were similar. Four protein spots were identified as being significantly different in the two groups. Two spots were overexpressed in the disomy 3 tumors while two spots were underexpressed. Identification of the four spots yielded nine proteins. Immunoblotting confirmed the results for heat shock protein 27, vimentin, and pyruvate dehydrogenase beta with a statistical significance for the first two proteins. In the monosomy 3 tumors heat shock protein 27 was down-regulated whilst vimentin was up-regulated.

Conclusions: : Heat shock protein 27 and vimentin differed significantly between tumours with disomy and monosomy 3. These proteins may play an important role in uveal melanoma metastasis.

Keywords: melanoma • uvea • proteomics 
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