May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
High Intraocular Pressure (IOP)-Induced Ischemia Elevates Extracellular Glutamate in the Retina of Rat: Pharmacological Evidence to Implicate Derangement of Glutamate Transporters
Author Affiliations & Notes
  • C. Nucci
    Univ of Rome Tor Vergata, Rome, Italy
    Ophthalmology; Mondino-Tor Vergata Center for Exp. Neuropharmacology,
  • A. Cerulli
    Univ of Rome Tor Vergata, Rome, Italy
    Ophthalmology,
    Pharmacobiology, University of Calabria, Cosenza, Italy
  • F. Cavaliere
    Pharmacobiology, University of Calabria, Cosenza, Italy
  • R. Russo
    Pharmacobiology, University of Calabria, Cosenza, Italy
  • L. Rombolà
    Pharmacobiology, University of Calabria, Cosenza, Italy
  • E. Fazzi
    University of Pavia; IRCCS C. Mondino Foundation, Pavia, Italy
  • G. Bagetta
    Pharmacobiology, University of Calabria, Cosenza, Italy
  • M. Corasaniti
    Faculty of Pharmacy, University of Catanzaro, Catanzaro, Italy
  • L. Morrone
    Pharmacobiology, University of Calabria, Cosenza, Italy
  • Footnotes
    Commercial Relationships  C. Nucci, None; A. Cerulli, None; F. Cavaliere, None; R. Russo, None; L. Rombolà, None; E. Fazzi, None; G. Bagetta, None; M. Corasaniti, None; L. Morrone, None.
  • Footnotes
    Support  Italian Health Ministry, Visufarma Italy
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 864. doi:https://doi.org/
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      C. Nucci, A. Cerulli, F. Cavaliere, R. Russo, L. Rombolà, E. Fazzi, G. Bagetta, M. Corasaniti, L. Morrone; High Intraocular Pressure (IOP)-Induced Ischemia Elevates Extracellular Glutamate in the Retina of Rat: Pharmacological Evidence to Implicate Derangement of Glutamate Transporters. Invest. Ophthalmol. Vis. Sci. 2008;49(13):864. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : to study by ocular microdialysis the extracellular changes of glutamate in high IOP-induced ischemia in vivo, in the presence of DL-threo-beta-benzyloxyaspartate (DL-TBOA), a non selective, non-transportable blocker of excitatory amino acid transporters (EAATs) or a solution (coenzyme Q10 (0.1%) and Vitamin E (0.5%) (CoQun, Visufarma, Italy)) with documented free-radical scavenger and anti-apoptotic properties (Nucci et al., 2007 Int. Rev. Neurobiol.).

Methods: : retinal ischemia was induced in the right eye of anaesthetised, male, Wistar rat (250 g) by acutely increasing the IOP (see Osborne et al., 2004, Prog Ret Eye Res) and extracellular glutamate monitored during and after ischemia using microdialysis (see Nucci et al., 2005, Neurotoxicology). DL-TBOA or Coenzyme Q10 + Vitamin E solution were administered intravitreally via the microdialysis probe.

Results: : extracellular glutamate increases during the first 10 min of ischemia though this reach statistical significace 10 min and 150 min after the reperfusion had started. Administration of DL-TBOA (500 µM) during ischemia showed a trend towards glutamate increase at 10 min after reperfusion and a more sustained glutamate increase after 150 min of reperfusion without reaching, however, statistical significance whereas Coenzyme Q10 + Vitamin E solution ablated the peak increases typically induced by ischemia-reperfusion sequence.

Keywords: neuroprotection • excitatory neurotransmitters • oxidation/oxidative or free radical damage 
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