May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Vitreous Pharmacokinetics and Retinal Toxicity Comparative Study of Preserved and Non Preserved Triamcinolone in Rabbits Eyes
Author Affiliations & Notes
  • R. C. Oliveira
    Ophthalmology - Retina, Univ Sao Paulo-Ribeirao Preto, Ribeirao Preto, Brazil
  • R. C. Siqueira
    Ophthalmology - Retina, Univ Sao Paulo-Ribeirao Preto, Ribeirao Preto, Brazil
  • A. S. C. Junior
    Faculdade de Farmacia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
  • A. Maia
    Faculdade de Veterinaria, Unirp, Sao Jose do Rio Preto, Brazil
  • H. Atique
    Faculdade de Veterinaria, Unirp, Sao Jose do Rio Preto, Brazil
  • F. M. Damico
    Ophthalmology - Retina, Univ Sao Paulo, Sao Paulo, Brazil
  • R. Jorge
    Ophthalmology - Retina, Univ Sao Paulo-Ribeirao Preto, Ribeirao Preto, Brazil
  • Footnotes
    Commercial Relationships  R.C. Oliveira, None; R.C. Siqueira, None; A.S.C. Junior, None; A. Maia, None; H. Atique, None; F.M. Damico, None; R. Jorge, None.
  • Footnotes
    Support  FAEPA
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 875. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      R. C. Oliveira, R. C. Siqueira, A. S. C. Junior, A. Maia, H. Atique, F. M. Damico, R. Jorge; Vitreous Pharmacokinetics and Retinal Toxicity Comparative Study of Preserved and Non Preserved Triamcinolone in Rabbits Eyes. Invest. Ophthalmol. Vis. Sci. 2008;49(13):875. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : The purpose of this study was to determine the intravitreous pharmacokinetic profile of benzyl alcohol triamcinolone acetonide formulation, and preservative-free triamcinolone acetonide formulation, and evaluate potential signs of toxicity to the retina.

Methods: : Fifty New Zealand male white rabbits, divided in two groups, were enrolled in the study. In group I, 25 rabbits received 4 mg (0,1 ml) of preservative benzyl alcohol triamcinolone acetonide formulation into the vitreous of the right eye. In group II, 25 rabbits received 4mg (0,1ml) of preservative-free triamcinolone. Five rabbits of each group was euthanized at 3, 7, 14, 21 e 28 days after injections, and intravitreal drug levels were determined by enzyme linked immunosorbent assay (ELISA). Ocular pressure of the experimental eye was measured preoperatively, postoperatively, and on 14 and 28 days after injection. Five animals in each group were randomly chosen 27 days after injection for light microscopy examination. Eletroretinography (ERG) was performed on both eyes of each five rabbits of group I and II at baseline and 27 days after injection.

Results: : At 7 days after injection, the mean triamcinolone intravitreous concentration in the group I was higher than group II. In the other days the mean concentration observed was the same in both groups. There was no evidence of toxic effects on both groups based on histological and ERG findings.

Conclusions: : Our findings showed that preservative benzyl alcohol triamcinolone acetonide formulation and preservative-free triamcinolone acetonide formulation had the different vitreous concentration just at seven days after intravitreous injection. No toxic reactions in the retina were observed in both groups.

Keywords: retina • inflammation • drug toxicity/drug effects 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×