May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Effect of Ketorolac on Rat Retinal Neurosensory Cells and Human Retinal Pigment Epithelial Cells in vitro
Author Affiliations & Notes
  • M. F. Estrago Franco
    Ophthalmology, Univ of California Irvine, Irvine, California
  • A. Jayaprakash Patil
    Ophthalmology, Univ of California Irvine, Irvine, California
  • A. Sharma
    Ophthalmology, Univ of California Irvine, Irvine, California
  • M. Chwa
    Ophthalmology, Univ of California Irvine, Irvine, California
  • G. M. Seigel
    Ophthalmology, Physiology and Biophysics, Ross Eye Institute, Buffalo, New York
  • M. C. Kenney
    Ophthalmology, Univ of California Irvine, Irvine, California
  • B. D. Kuppermann
    Ophthalmology, Univ of California Irvine, Irvine, California
  • Footnotes
    Commercial Relationships  M.F. Estrago Franco, None; A. Jayaprakash Patil, None; A. Sharma, None; M. Chwa, None; G.M. Seigel, None; M.C. Kenney, None; B.D. Kuppermann, None.
  • Footnotes
    Support  Discovery Eye Foundation, Gilbert Foundation, the Iris and B. Gerald Cantor Foundation and Research to Prevent Blindness Foundation.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 876. doi:https://doi.org/
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      M. F. Estrago Franco, A. Jayaprakash Patil, A. Sharma, M. Chwa, G. M. Seigel, M. C. Kenney, B. D. Kuppermann; Effect of Ketorolac on Rat Retinal Neurosensory Cells and Human Retinal Pigment Epithelial Cells in vitro. Invest. Ophthalmol. Vis. Sci. 2008;49(13):876. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To study the effect of 0.4% Ketorolac tromethamine ophthalmic solution (Acular LS, Allergan, Irvine, California), a topical nonsteroidal anti-inflammatory drug, on human retinal pigment epithelial (ARPE-19) and rat retinal neurosensory (R28) cells in vitro.

Methods: : ARPE-19 and R28 cells were grown in tissue culture in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum. Cells were treated with five different concentrations of Ketorolac (300 µg/ml, 200 µg/ml, 100 µg/ml , 50 µg/ml and 25 µg/ml) for 24 hours. The doses were chosen to bracket the intravitreal clinical dose determined by assuming 0.1 cc of the commercial preparation distributes equally throughout the 4 ml human vitreous volume. Using this approach the calculated clinical dose is 100 µg/ml. Toxicity was determined by a trypan blue dye-exclusion cell viability assay.

Results: : The mean cell viability of ARPE-19 cells after 24 hours exposure to 300 µg/ml, 200 µg/ml, 100 µg/ml, 50 µg/ml and 25 µg/ml of Ketorolac was: 38.31% ± 11.29 (P < 0.001), 82.4% ± 9.2 (P > 0.05), 96.4% ± 1.42 (P > 0.05), 95.8% ± 2.03 (P > 0.05) and 94.8% ± 3.5 (P > 0.05) respectively, compared to untreated control ARPE-19 cells that had a viability of 96.28 ± 1.5. The mean cell viability of R28 cells exposed to 24 hours of 300 µg/ml, 200 µg/ml, 100 µg/ml, 50 µg/ml and 25 µg/ml of Ketorolac was: 64.4% ± 12.5 (P < 0.001), 93.1% ± 2.7 (P > 0.05), 91.6% ± 3.7 (P > 0.05), 91.3% ± 3.7 (P > 0.05) and 90.5% ± 3.7 (P > 0.05) respectively, compared to untreated control R28 cells that had a viability of 89.46 ± 5.11.

Conclusions: : These results suggest that Ketorolac at clinically relevant intravitreal doses is not toxic to ARPE-19 and R28 cells in vitro.

Keywords: drug toxicity/drug effects • cell survival • retinal culture 
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