May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Cellular Specificity of Expression After Subretinal Injection of Fetal Murine Retina Is Dependent on Aav Dose
Author Affiliations & Notes
  • D. C. Chung
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • V. Lee
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • Z. Wei
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • J. Bennett
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • Footnotes
    Commercial Relationships  D.C. Chung, None; V. Lee, None; Z. Wei, None; J. Bennett, None.
  • Footnotes
    Support  NIH Grants K08 EY017024, RO1 EY10820, P30-DK-47747-10, Foundation Fighting Blindness, Research to Prevent Blindness, the Paul and Evanina Mackall Foundation Trust and the F.M. Kirby Foundation
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 877. doi:https://doi.org/
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    • Get Citation

      D. C. Chung, V. Lee, Z. Wei, J. Bennett; Cellular Specificity of Expression After Subretinal Injection of Fetal Murine Retina Is Dependent on Aav Dose. Invest. Ophthalmol. Vis. Sci. 2008;49(13):877. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Methods: : Wild-type C57Bl6 mice underwent timed pregnancies to determine the date of conception. At embryonic day 14 (E14), the left subretinal space was injected with an AAV (AAV.CMV.eGFP) carrying the enhanced green fluorescent protein marker gene driven by the CMV promoter. Viral concentrations used spanned a range of 1.2 log unit, with the largest dose administered being 3.2E8 gc Contralateral eyes were uninjected. At postnatal day 28, animals were euthanized, eyes enucleated, cryoprotected and sectioned. Retinal sections were examined on a fluorescent microscope for GFP expression.

Results: : Eyes injected with the lowest doses of AAV showed expression exclusively in the retinal pigmented epithelium (RPE). After injection of the higher doses, expression was evident in RPE and photoreceptors. No toxicity was appreciated.

Keywords: gene transfer/gene therapy • retina • gene/expression 
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