May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Hyperspectral Imaging: A Non-Invasive System for Visualizing Retinal Oxygen Saturation in Humans
Author Affiliations & Notes
  • J. Elison
    Ophthalmology, LSU Department of Ophthalmology, New Orleans, Louisiana
  • H. Toma
    Ophthalmology, LSU Department of Ophthalmology, New Orleans, Louisiana
  • B. Khoobehi
    Ophthalmology, LSU Department of Ophthalmology, New Orleans, Louisiana
  • R. Grigorian
    Ophthalmology, LSU Department of Ophthalmology, New Orleans, Louisiana
  • E. Puissegur
    Ophthalmology, LSU Department of Ophthalmology, New Orleans, Louisiana
  • Footnotes
    Commercial Relationships  J. Elison, None; H. Toma, None; B. Khoobehi, None; R. Grigorian, None; E. Puissegur, None.
  • Footnotes
    Support  NEI R03EY012887 (BK), P30EY02377 (LSU Eye Center Core grant), and an unrestricted challenge grant from Research to Prevent Blindness, New York, New York (LSU Eye Center).
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 893. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J. Elison, H. Toma, B. Khoobehi, R. Grigorian, E. Puissegur; Hyperspectral Imaging: A Non-Invasive System for Visualizing Retinal Oxygen Saturation in Humans. Invest. Ophthalmol. Vis. Sci. 2008;49(13):893. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose:
 

To use a hyperspectral imaging (HSI) system as a non-invasive method to measure oxygen delivery to the optic nerve head (ONH) and retinal vessels in human subjects.

 
Methods:
 

Fundus images of the optic nerve head, retina, and their overlying vessels were obtained from healthy human subjects using the HSI system. Reflectance light spectroscopy under constant illumination of ONH and retinal areas was performed with HSI. Reflectance oxyhemoglobin and deoxyhemoglobin spectral curves were generated from light reflected from each region of interest at wavelengths ranging from 409 nm to 917 nm. A predetermined algorithm was used to calculate the relative oxygen saturation indices from the different structures.

 
Results:
 

The relative saturation indices for the arteriole, venule, and ONH areas were found to be as follows: 3.1, 2.4, and 4.7.

 
Conclusions:
 

This study non-invasively measures oxygen saturation levels in the eyes of human subjects. We believe that this novel non-invasive approach to measuring the oxygen levels in the ocular structures of human subjects will contribute to our understanding of how oxygen is provided and used by the eye.  

 
Keywords: oxygen • retina • imaging/image analysis: clinical 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×