May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Localization of Vascular Adhesion Protein-1 in Human Ocular Tissues
Author Affiliations & Notes
  • A. Hafezi-Moghadam
    Ophthalmology, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts
  • L. Almulki
    Ophthalmology, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts
  • K. Noda
    Ophthalmology, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts
  • T. Hisatomi
    Ophthalmology, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts
  • S. Nakao
    Ophthalmology, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts
  • Y. Mashima
    R-Tech Ueno, Ltd., Tokyo, Japan
  • S. Mallemadugula
    Ophthalmology, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts
  • F. Tayyari
    Ophthalmology, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts
  • N. Michaud
    Ophthalmology, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts
  • K. L. Thomas
    Ophthalmology, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  A. Hafezi-Moghadam, RTECH UENO, F; RTECH UENO, C; L. Almulki, None; K. Noda, None; T. Hisatomi, None; S. Nakao, None; Y. Mashima, RTECH UENO, E; S. Mallemadugula, None; F. Tayyari, None; N. Michaud, None; K.L. Thomas, None.
  • Footnotes
    Support  RTECH UENO, RPB, NIH grant AI050775 to A.H.-M., NEI core grant EY14104, Massachusetts Lions Foundation, and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 950. doi:https://doi.org/
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      A. Hafezi-Moghadam, L. Almulki, K. Noda, T. Hisatomi, S. Nakao, Y. Mashima, S. Mallemadugula, F. Tayyari, N. Michaud, K. L. Thomas; Localization of Vascular Adhesion Protein-1 in Human Ocular Tissues. Invest. Ophthalmol. Vis. Sci. 2008;49(13):950. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Recently we showed a critical role for Vascular Adhesion Protein-1 (VAP-1) in a model of uveitis. VAP-1 inhibition ameliorated signs of acute inflammation, including retinal leukostasis and cytokine production in EIU animals. However, until now the expression of VAP-1 in the human eye was not known.

Methods: : Paraffin blocks of human ocular tissues were obtained from MEEI’s tissue sample archives. VAP-1 tissue localization was assessed in 5 µm thick sections using immunohistochemistry. Sections were incubated with primary mAbs against VAP-1 (5 µg/ml), smooth muscle actin (1 µg/ml), or isotype matched IgG at 4 °C overnight. Subsequently, a secondary antibody (mouse envision system) was used for 30 min at room temperature followed by Dako Envision + HRP (AEC) System for signal detection. The stained sections were examined using light microscopy and the signal intensity was quantified by two masked evaluators and graded into 4 different categories, non-detectable (0) to higher intensities (1, 2 and 3).

Results: : Within various ocular tissues, VAP-1 staining was confined to the vasculature. VAP-1 labeling showed the highest intensity in both arteries and veins of neuronal tissues, retina and optic nerve, and the lowest intensity in the iris vasculature. Scleral and choroidal vessels showed moderate staining for VAP-1. VAP-1 intensity was significantly higher in the arteries compared to veins. Furthermore, VAP-1 staining in arteries co-localized with SM-actin staining, suggesting expression of VAP-1 in smooth muscle cells or potentially pericytes.

Conclusions: : Immunohistochemistry reveals a constitutive expression of VAP-1 in human ocular tissues. VAP-1 expression is exclusive to the vasculature with arteries showing higher expression than veins. Furthermore, the vessels of the optic nerve and the retina show the highest level of expression. These and our previous results suggest VAP-1 to be a key molecule in ocular vascular and inflammatory diseases.

Keywords: age-related macular degeneration • uveitis-clinical/animal model • diabetic retinopathy 
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