May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Interaction Between Vitreous-Derived Cells and Vascular Endothelial Cells in Vitreoretinal Diseases
Author Affiliations & Notes
  • N. Tojo
    Dept of Ophthal and Visual Science, Yamagata University Sch of Med, Yamagata, Japan
    Department of Ophthalmology Visual Science, Chiba University Graduate School of Medicine, Chiba, Japan
  • Y. Kashiwagi
    Department of Ocular Cellar Engineering, Yamagata University Hospital, Yamagata, Japan
  • K. Nishitsuka
    Dept of Ophthal and Visual Science, Yamagata University Sch of Med, Yamagata, Japan
  • S. Yamamoto
    Department of Ophthalmology Visual Science, Chiba University Graduate School of Medicine, Chiba, Japan
  • H. Asao
    Department of Immunology, Yamagata University School of Medicine, Yamagata, Japan
  • N. Sugawara
    Mitsubishi Medience, Tokyo, Japan
  • T. Yamashita
    Mitsubishi Medience, Tokyo, Japan
  • T. Yamamoto
    Department of Ocular Cellar Engineering, Yamagata University Hospital, Yamagata, Japan
  • H. Yamashita
    Dept of Ophthal and Visual Science, Yamagata University Sch of Med, Yamagata, Japan
  • Footnotes
    Commercial Relationships  N. Tojo, None; Y. Kashiwagi, None; K. Nishitsuka, None; S. Yamamoto, None; H. Asao, None; N. Sugawara, None; T. Yamashita, None; T. Yamamoto, None; H. Yamashita, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 951. doi:
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    • Get Citation

      N. Tojo, Y. Kashiwagi, K. Nishitsuka, S. Yamamoto, H. Asao, N. Sugawara, T. Yamashita, T. Yamamoto, H. Yamashita; Interaction Between Vitreous-Derived Cells and Vascular Endothelial Cells in Vitreoretinal Diseases. Invest. Ophthalmol. Vis. Sci. 2008;49(13):951.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Methods: : Human retinal endothelial cells, human umbilical vein endothelial cells (HUVEC) and porcine vitreous-derived cells (Nishitsuka K, et al. Exp Eye Res 2007;85:539-545.) were used. The expression of vascular endothelial growth factor (VEGF) or interleukin 6 (IL-6) at the mRNA and protein levels, was observed with PCR and ELISA respectively. The effects on vascular endothelial cells by vitreous-derived cells were inquired in a co-culture system with or without exposure to IL-1α(Interleukin-1alpha), IL-1β (Interleukin-1beta), IL-6, TNFα(tumor necrosis factor alpha) or VEGF. The effects on vascular endothelial cells were assessed by MTT assay and tube formation.

Results: : The expression levels of VEGF and IL-6 were increased both at mRNA and protein levels by the treatment with IL-1α, IL-1β , or TNFα, but not with VEGF or IL-6 in the vitreous-derived cells. The vascular endothelial cells were stimulated to proliferate by VEGF and IL-6. The viability of human retinal endothelial cells was affected in a co-culture system with vitreous-derived cells in the presence with IL-1α, IL-1β , IL-6, TNFα, and VEGF. The tube formation using HUVEC was accelerated by IL-6 or VEGF.

Keywords: vitreous • neovascularization • inflammation 
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