May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Bacterial Contamination Rate of the Anterior Chamber During Cataract Surgery Using Conventional Culture and Eubacterial PCR
Author Affiliations & Notes
  • P.-L. Cornut
    Ophthalmology, Herriot Hospital-Lyon France, Lyon, France
  • F. Vandenesch
    Microbiology,
    CHU-Lyon France, Lyon, France
  • G. Lina
    Microbiology,
    CHU-Lyon France, Lyon, France
  • Y. Benito
    Microbiology,
    CHU-Lyon France, Lyon, France
  • J. Etienne
    Microbiology,
    CHU-Lyon France, Lyon, France
  • C. Piras
    Ophthalmology, Herriot Hospital-Lyon France, Lyon, France
  • L. Kodjikian
    Ophthalmology,
    CHU-Lyon France, Lyon, France
  • C. Burillon
    Ophthalmology, Herriot Hospital-Lyon France, Lyon, France
  • P. Denis
    Ophthalmology, Herriot Hospital-Lyon France, Lyon, France
  • Footnotes
    Commercial Relationships  P. Cornut, None; F. Vandenesch, None; G. Lina, None; Y. Benito, None; J. Etienne, None; C. Piras, None; L. Kodjikian, None; C. Burillon, None; P. Denis, None.
  • Footnotes
    Support  Société Française d'Ophtalmologie
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 954. doi:
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      P.-L. Cornut, F. Vandenesch, G. Lina, Y. Benito, J. Etienne, C. Piras, L. Kodjikian, C. Burillon, P. Denis; Bacterial Contamination Rate of the Anterior Chamber During Cataract Surgery Using Conventional Culture and Eubacterial PCR. Invest. Ophthalmol. Vis. Sci. 2008;49(13):954.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the incidence of anterior chamber bacterial contamination during phacoemulsification cataract surgery using eubacterial polymerase chain reaction and conventional cultures.

Methods: : This prospective study included 30 eyes of 24 patients who had phacoemulsification with implantation of a foldable acrylic posterior chamber intraocular lens, through a 3.2-mm clear corneal incision. Topical aminosid was administrated 3 days before surgery. After povidone-iodine antisepsis, two intraoperative anterior chamber aspirates were obtained from each patient, the first taken upon entering the anterior chamber and the second at the conclusion of surgery after the suture. Broad-range eubacterial PCR amplification and conventional cultures were used to verify that aqueous humor did not contain any detectable bacteria at the beginning of the surgery and to evaluate the bacterial contamination rate of the anterior chamber at the end of it. No oral antibiotic prophylaxis was used.

Results: : No specimens (0%) aspirated on entry into the anterior chamber or obtained at the conclusion of surgery were positive for microorganisms on culture or eubacterial PCR. None of the eyes developed acute endophthalmitis.

Conclusions: : The incidence of anterior chamber bacterial contamination during phacoemulsification recovered in this study using eubacterial PCR and conventional culture was null (0%).

Keywords: bacterial disease • cataract • microbial pathogenesis: clinical studies 
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