May 2008
Volume 49, Issue 13
ARVO Annual Meeting Abstract  |   May 2008
Synaptic Expression of APOER2 in the Plexiform Layers of Mouse Retina
Author Affiliations & Notes
  • J. Ding
    Duke University Medical Center, Durham, North Carolina
  • C. Bowes Rickman
    Duke University Medical Center, Durham, North Carolina
    Cell Biology,
  • Footnotes
    Commercial Relationships  J. Ding, None; C. Bowes Rickman, None.
  • Footnotes
    Support  NEI R21 EY01712, RPB core grant
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1292. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J. Ding, C. Bowes Rickman; Synaptic Expression of APOER2 in the Plexiform Layers of Mouse Retina. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1292. doi:

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : Apolipoprotein E receptor 2 (APOER2), a member of LDL receptor family, is highly expressed in the brain and plays an important role in synaptic plasticity and neurodegeneration. However, its involvement in retinal synaptic transmission is not clear. In this study, we tested whether APOER2 is also involved in retinal synaptic transmission by first examining whether it is expressed in retinal synapses.

Methods: : We performed immunohistochemistry to study the distribution of APOER2 in mouse retina. We also used double labeling of APOER2 with a presynaptic marker, SV2, and confocal microscopy to investigate its expression in retinal synapses. Calbindin and PKCα were used to identify postsynaptic processes of horizontal cells and rod bipolar cells, respectively.

Results: : Anti-APOER2 antibody (NOVUS) exhibited a punctate staining pattern in mouse retina, prominent in both the outer plexiform layer (OPL) and inner plexiform layer (IPL). In the OPL, APOER2-positive puncta were not colocalized with SV2-positive photoreceptor terminals, but enclosed within them. These puncta were not colocalized with calbindin, but some of them colocalized with PKCα, suggesting they are expressed by postsynaptic rod bipolar dendrites. In the IPL, APOER2-positive puncta were also closely apposed to SV2-positive puncta, suggesting postsynaptic expression.

Conclusions: : In mouse retina APOER2 expression is closely associated with synapses. We will further clarify if APOER2 is expressed in pre- or postsynaptic compartment using immunoelectron microscopy. We will also identify the retinal cells that express APOER2 by multiple-labeling with different cellular markers.

Keywords: receptors • synapse • immunohistochemistry 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.