May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Substance P and Calcitonine Gene-Related Peptide Intrinsic Choroidal Neurons in Human Choroidal Whole-Mounts
Author Affiliations & Notes
  • R. de Hoz
    Inst Inves Oftalmol Ramon Castroviejo, Univ Complutense de Madrid, Madrid, Spain
  • B. Rojas
    Inst Inves Oftalmol Ramon Castroviejo, Univ Complutense de Madrid, Madrid, Spain
  • J. J. Salazar
    Inst Inves Oftalmol Ramon Castroviejo, Univ Complutense de Madrid, Madrid, Spain
  • A. I. Ramirez
    Inst Inves Oftalmol Ramon Castroviejo, Univ Complutense de Madrid, Madrid, Spain
  • A. Redondo
    Inst Inves Oftalmol Ramon Castroviejo, Univ Complutense de Madrid, Madrid, Spain
  • J. M. Ramirez
    Inst Inves Oftalmol Ramon Castroviejo, Univ Complutense de Madrid, Madrid, Spain
  • A. Triviño
    Inst Inves Oftalmol Ramon Castroviejo, Univ Complutense de Madrid, Madrid, Spain
  • Footnotes
    Commercial Relationships  R. de Hoz, None; B. Rojas, None; J.J. Salazar, None; A.I. Ramirez, None; A. Redondo, None; J.M. Ramirez, None; A. Triviño, None.
  • Footnotes
    Support  Mº Educacion y Ciencia (Spain) SAF2006-05955
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1313. doi:https://doi.org/
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    • Get Citation

      R. de Hoz, B. Rojas, J. J. Salazar, A. I. Ramirez, A. Redondo, J. M. Ramirez, A. Triviño; Substance P and Calcitonine Gene-Related Peptide Intrinsic Choroidal Neurons in Human Choroidal Whole-Mounts. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1313. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine the presence of substance P (SP)-and calcitonine gene-related peptide (CGRP) positive intrinsic choroidal neurons (ICNs) in human.

Methods: : Nineteen choroidal whole-mounts were processed for indirect immunofluorescence. Antibody to a component of the neuronal cytoskeleton, neurofilament 200 kDa (NF-200), was combined with antibodies to SP and to CGRP (neuropeptides proper to the sensory nervous system).

Results: : The human choroid possesses numerous SP(+) and CGRP(+) ICNs. These neurons were observed in the suprachoroid, both in isolation and forming microganglia. For both types of ICNs studied, neurons were more numerous in the temporal than in the nasal regions. In both locations, SP(+) and CGRP(+) ICNs were more abundant in the central choroid (the choroid underneath the macular area of the retina), with cell density diminishing outwards to the choroidal periphery. There were no appreciable differences between the two populations of ICNs studied in terms of size, morphology or immunostaining characteristics.

Conclusions: : The human choroid contains an abundant population of SP(+) and CGRP(+) ICNs. Given that peripheral sensory innervation could be involved in the regulation of both choroidal blood flow and vascular architecture, the SP(+) and CGRP(+) ICNs described for first time in the present work may be implicated in these mechanisms of vascular regulation.

Keywords: innervation: sensation • choroid • microscopy: light/fluorescence/immunohistochemistry 
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