May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Increased Corneal Angiogenesis in the Fhl2 Knockout Mice
Author Affiliations & Notes
  • L.-K. Yeh
    Chang Gung Memorial Hospital, Taipei, Taiwan
    Department of Ophthalmology,
  • P.-H. Chua
    Chang Gung Memorial Hospital, Taipei, Taiwan
    Department of Cardiology,
  • I.-J. Wang
    Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
  • Footnotes
    Commercial Relationships  L. Yeh, None; P. Chua, None; I. Wang, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1322. doi:https://doi.org/
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      L.-K. Yeh, P.-H. Chua, I.-J. Wang; Increased Corneal Angiogenesis in the Fhl2 Knockout Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1322. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The aim of the present work is to investigate the issue of corneal angiogenesis in ocular surfaces. We evaluated the increased corneal angiogenesis in the cardiac- specific muscle LIM protein FHL2 deficiency mice.

Methods: : We compared the corneal angiogenesis between the FHL2 knockout mice and wild type mice by using the chemical (NaOH) injury in the central corneal area. After soaking NaOH for 1 minute, the injuried cornea was washed by PBS buffered. The new growth vessels were observed and recorded by dissecting microscope. The VEGF and Cox-2 were evaluated by the methods of immunohistochemistry and western blot.

Results: : Significant increased corneal vessels were formed on the FHL2 knockout mice. The western blot results showed that the VEGF and Cox-2 protein were upregulated by the chemical injury in the FHL2 knockout mice. Immunohistocytochemistry showed that the VEGF and Cox-2 protein were over-expressed on the corneal tissue.

Conclusions: : We conclude that the increased inflammatory corneal angiogenesis in the FHL2 knockout mice by the chemical injury.

Keywords: cornea: basic science • neovascularization • inflammation 
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