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L.-K. Yeh, P.-H. Chua, I.-J. Wang; Increased Corneal Angiogenesis in the Fhl2 Knockout Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1322. doi: https://doi.org/.
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The aim of the present work is to investigate the issue of corneal angiogenesis in ocular surfaces. We evaluated the increased corneal angiogenesis in the cardiac- specific muscle LIM protein FHL2 deficiency mice.
We compared the corneal angiogenesis between the FHL2 knockout mice and wild type mice by using the chemical (NaOH) injury in the central corneal area. After soaking NaOH for 1 minute, the injuried cornea was washed by PBS buffered. The new growth vessels were observed and recorded by dissecting microscope. The VEGF and Cox-2 were evaluated by the methods of immunohistochemistry and western blot.
Significant increased corneal vessels were formed on the FHL2 knockout mice. The western blot results showed that the VEGF and Cox-2 protein were upregulated by the chemical injury in the FHL2 knockout mice. Immunohistocytochemistry showed that the VEGF and Cox-2 protein were over-expressed on the corneal tissue.
We conclude that the increased inflammatory corneal angiogenesis in the FHL2 knockout mice by the chemical injury.
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