May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Fate Mapping for Neural Crest Cells During Ocular Development With Protein 0 Promoter-Driven Transgenic Technique
Author Affiliations & Notes
  • M. Inatani
    Dept of Ophthalmol & Vis Sci, Kumamoto Univ Grad Sch of Med Sci, Kumamoto, Japan
  • K. Iwao
    Dept of Ophthalmol & Vis Sci, Kumamoto Univ Grad Sch of Med Sci, Kumamoto, Japan
    Department of Ophthalmology, Faculty of Medicine, Saga University, Nabeshima, Japan
  • S. Okinami
    Department of Ophthalmology, Faculty of Medicine, Saga University, Nabeshima, Japan
  • H. Tanihara
    Dept of Ophthalmol & Vis Sci, Kumamoto Univ Grad Sch of Med Sci, Kumamoto, Japan
  • Footnotes
    Commercial Relationships  M. Inatani, None; K. Iwao, None; S. Okinami, None; H. Tanihara, None.
  • Footnotes
    Support  KAKENHI Young Scientists (S) 19679008
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1323. doi:https://doi.org/
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      M. Inatani, K. Iwao, S. Okinami, H. Tanihara; Fate Mapping for Neural Crest Cells During Ocular Development With Protein 0 Promoter-Driven Transgenic Technique. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1323. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the fate map of neural crest cells tracked by Protein 0 promoter-driven Cre recombinase during mammalian ocular development.

Methods: : The neural crest cells in mice developing eyes (between embryonic day 8.5 and postnatal day 42) were traced by a complementally binary genetic technique using a transgene expressing the Cre recombinase under the control of Protein 0 promoter and a Rosa26 Cre-responsive reporter gene producing β-galactosidase (β-gal) after Cre-mediated recombination.

Results: : β-gal-positive cells were detectable in periocular segment at E9.5. In the late embryonic stages (E13.5 to E18.5), neural crest cells-derived tissues including corneal stroma, corneal endothelium, iridocorneal angle, ciliary body, primary vitreous and eyelid were intensely stained. The intensity of β-gal-staining was gradually decreased in the corneal stroma after the birth while β-gal-positive cells were densely localized in the presumptive area of the iridocorneal angle throughout the ocular development.

Conclusions: : These results demonstrate that Protein 0-Cre transgenic mice provide the fate of neural crest cells in ocular anterior segment as well as the potential for conditional knocked-out strategy about the candidate molecules for the differentiation of the ocular anterior segment.

Keywords: anterior segment • ciliary body • cornea: endothelium 
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