May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Expression of GFAP and RAGE in the Retina of Three Different Diabetic Rat Models
Author Affiliations & Notes
  • J. E. Mancini
    Ophthalmology, facultad de Ciencias Biomedicas, Buenos Aires, Argentina
  • J. C. Basabe
    Endocrinology, Centro de Investigaciones Endocrinológicas, Buenos Aires, Argentina
  • O. J. Croxatto
    Ophthalmolmic Pathology, Ophthalmolmic Pathology, Fundación Oftalmológica Argentina Jorge Malbran", Buenos Aires, Argentina
  • J. E. Gallo
    Ophthalmology, facultad de Ciencias Biomedicas, Buenos Aires, Argentina
  • Footnotes
    Commercial Relationships  J.E. Mancini, None; J.C. Basabe, None; O.J. Croxatto, None; J.E. Gallo, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1326. doi:https://doi.org/
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      J. E. Mancini, J. C. Basabe, O. J. Croxatto, J. E. Gallo; Expression of GFAP and RAGE in the Retina of Three Different Diabetic Rat Models. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1326. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : to compare the immunohistochemical expression of GFAP and RAGE in the retina of three different models of experimental diabetes.

Methods: : Type 2 diabetes models were constituted by two groups of four male Wistar neonatal rats that received an intraperitoneal injection of 90 mg/kg and 45 mg/kg streptozotocin (SZT) at day 2 of life, respectively. Rats with lower doses of STZ were fed by a high fat diet (HFD) from week 8 to sacrifice. Type 1 model was made up of adult male rats treated with an intraperitoneal injection of 45 mg/kg STZ. All animals, including control non-diabetic group, were sacrificed at 37 weeks of diabetes. Retinas were processed and analysed by immunohistochemical techniques using GFAP and RAGE antibodies.

Results: : The immunoreactivity of GFAP found in the fiber layer of diabetic animals was much higher than that seen in controls. In addition, staining of the fiber layer and Muller like-cells was stronger in type 1 diabetics. RAGE expression was seen in the fiber layer and ganglion cell layer of diabetic rats. However, a positive immunoreactivity in the internal nuclear layer was observed in type 1 and type 2 diabetic rats with HFD.

Conclusions: : Different localization and intensity of the expression of GFAP and RAGE in type 1 and type 2 diabetic rats could be useful to better know the process of oxidative stress and oxidation in the pathophysiology of diabetic retinopathy.

Keywords: diabetic retinopathy • retina • retinal degenerations: cell biology 
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