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J. E. Mancini, J. C. Basabe, O. J. Croxatto, J. E. Gallo; Expression of GFAP and RAGE in the Retina of Three Different Diabetic Rat Models. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1326. doi: https://doi.org/.
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to compare the immunohistochemical expression of GFAP and RAGE in the retina of three different models of experimental diabetes.
Type 2 diabetes models were constituted by two groups of four male Wistar neonatal rats that received an intraperitoneal injection of 90 mg/kg and 45 mg/kg streptozotocin (SZT) at day 2 of life, respectively. Rats with lower doses of STZ were fed by a high fat diet (HFD) from week 8 to sacrifice. Type 1 model was made up of adult male rats treated with an intraperitoneal injection of 45 mg/kg STZ. All animals, including control non-diabetic group, were sacrificed at 37 weeks of diabetes. Retinas were processed and analysed by immunohistochemical techniques using GFAP and RAGE antibodies.
The immunoreactivity of GFAP found in the fiber layer of diabetic animals was much higher than that seen in controls. In addition, staining of the fiber layer and Muller like-cells was stronger in type 1 diabetics. RAGE expression was seen in the fiber layer and ganglion cell layer of diabetic rats. However, a positive immunoreactivity in the internal nuclear layer was observed in type 1 and type 2 diabetic rats with HFD.
Different localization and intensity of the expression of GFAP and RAGE in type 1 and type 2 diabetic rats could be useful to better know the process of oxidative stress and oxidation in the pathophysiology of diabetic retinopathy.
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