Purchase this article with an account.
F. Yuan, Y.-Z. Yuan, L. Li; Stromal Cell-Derived Factor-1 Detected in the Diabetic Retina of Rat. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1331.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate the expression of stromal cell-derived factor-1 (SDF-1) in the experimental diabetic retina in rat.
6 to 8 week-old healthy male wistar rats for this study. 14 rats as the non-diabetic controls, and 45 made for diabetes mellitus groups induced by streptozotocin (STZ). The diabetic rats were raised for 2 months (DM2M) or 3 months (DM3M), respectively. The retinas of DM2M, DM3M and CON were fixed by 4% paraformaldehyde and embedded in paraffin, then checked by immunochemistry and digital photomicrograph analysis to detect the locations and levels of the expression of SDF-1. The ratios of integrated optical density (IOD) and area of interest (AOI) were calculated to evaluate the intensities of positive staining. Values of z, t and P were determined by Wilcoxon-Mann-Whitney test and Student’s t test). The SDF-1 mRNA of the retinas from DM2M and CON was detected by real-time RT-PCR. Copies of SDF-1 mRNA were then adjusted by the copies of β-actin mRNA in each sample.
The positive of SDF-1 in immunohistochemical staining was checked in all groups, which were located at the INL and GCL. There was no significant difference between DM2M and DM3M. Copies of SDF-1 mRNA were found in DM2M more than in the control, and the β-actin were highly express in DM2M than in normal control group.
SDF-1 was expressed highly in the diabetic retinas of rats, compared with normal control, suggesting it act as one of the pathogenesis of diabetic retinopathy.
This PDF is available to Subscribers Only