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L. Li, X. Cao, F. Yuan; The Effect of Triamcinolone Acetonide on Blood-Retinal Barrier Permeability and Retinal Occludin Expression in Diabetic Rats. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1346.
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to investigate the effect of intravitreal injection of triamcinolone acetonide (TA)on blood-retinal barrier(BRB) permeability and retinal occluding expression in diabetic rats.
eighty-four Sprague-Dawley rats were randomly divided into diabetic group and normal control group. Diabetes was induced by intraperitoneal injection of streptozotocin. After weeks, diabetic and normal rats were randomly divided into two groups: TA-injected group and balanced salt solution (BSS)-injected group. TA (2mg/8ul) or BSS was injected intravitreally in both diabetic group and normal control grous. Three, 7, 14 and 28 days after TA treatment and 7 and 28 days after BSS treatment, BRB leakage was assessed with Evans blue and retinal occludin expression was evaluated by western blot.
in normal control rats, there was no statistically significant difference of retinal Evans blue leakage and retinal occludin expression between TA-treated and BSS-treated group (P>0.05). In diabetic rats, retinal Evans blue leakage of TA-treated group was lower than that of in BSS-treated group(F=75.60 P<0.01). In diabetic rats, a marked increase of retinal occluding expression was also observed in TA-treated group, compared with BSS-treated group( F=56.36 P<0.01).
intravitreal injection of triamcinolone acetonide can reduce blood-retinal barrier permeability and promote retinal occludin expression in diabetic rats. The reduction of BRB permeability in diabetic rats by intravitreal injection of TA seems to be mediated via retinal occludin expression up-regulation.
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