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K. Saito, T. Sekiryu, I. Maruko, T. Iida; The Classification of the Pigment-Containing Cells in Surgically Excised Choroidal Neovascular Membranes. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1367.
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The purpose of this study was to classify the pigment-containing cells (PCC) in surgically excised choroidal neovascular membranes (CNVMs) and to investigate the vascular endothelial growth factor (VEGF) immunoreactivity.
Immunohistochemical analysis was performed on paraffin-embedded sections of surgically excised CNVMs from eight eyes (eight patients) with age-related macular degeneration (ARMD). Light microscopic immunohistochemical analysis was performed to detect Cytokeratin, CD68, and VEGF.
PCCs in CNVMs were classified into three groups. PCCs in the retinal pigment epithelium (RPE) layer were Cytokeratine-positive, CD68-negative, and VEGF-negative. PCCs on the suface of CNVMs were Cytokeratine-negative, CD68-negative, and VEGF-positive. The rest of PCCs were Cytokeratin-negative, CD68-positive, and VEGF-negative.
We have classified the immunohistchemical staining pattern of PCCs in surgically excised CNVMs. PCCs in the RPE layer were Cytokeratine- positive, and VEGF-negative. The rest of PCCs in CNVMs were clearly Cytokeratine-negative and were separated CD68-negative cells from CD68- positive cells. CD68-negative cells were VEGF-positive and located on the suface of CNVMs. CD68-positive cells were VEGF-negative and seen adjacent to the RPE layer or CD68-positive cells. RPE in CNVMs may loose characteristics of epithelium and perhaps become VEGF-positive or CD68-positive according to their roles.
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