May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
The Classification of the Pigment-Containing Cells in Surgically Excised Choroidal Neovascular Membranes
Author Affiliations & Notes
  • K. Saito
    Dept of Ophthalmology, Fukushima Medical University, Fukushima, Japan
  • T. Sekiryu
    Dept of Ophthalmology, Fukushima Medical University, Fukushima, Japan
  • I. Maruko
    Dept of Ophthalmology, Fukushima Medical University, Fukushima, Japan
  • T. Iida
    Dept of Ophthalmology, Fukushima Medical University, Fukushima, Japan
  • Footnotes
    Commercial Relationships  K. Saito, None; T. Sekiryu, None; I. Maruko, None; T. Iida, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1367. doi:https://doi.org/
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      K. Saito, T. Sekiryu, I. Maruko, T. Iida; The Classification of the Pigment-Containing Cells in Surgically Excised Choroidal Neovascular Membranes. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1367. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The purpose of this study was to classify the pigment-containing cells (PCC) in surgically excised choroidal neovascular membranes (CNVMs) and to investigate the vascular endothelial growth factor (VEGF) immunoreactivity.

Methods: : Immunohistochemical analysis was performed on paraffin-embedded sections of surgically excised CNVMs from eight eyes (eight patients) with age-related macular degeneration (ARMD). Light microscopic immunohistochemical analysis was performed to detect Cytokeratin, CD68, and VEGF.

Results: : PCCs in CNVMs were classified into three groups. PCCs in the retinal pigment epithelium (RPE) layer were Cytokeratine-positive, CD68-negative, and VEGF-negative. PCCs on the suface of CNVMs were Cytokeratine-negative, CD68-negative, and VEGF-positive. The rest of PCCs were Cytokeratin-negative, CD68-positive, and VEGF-negative.

Conclusions: : We have classified the immunohistchemical staining pattern of PCCs in surgically excised CNVMs. PCCs in the RPE layer were Cytokeratine- positive, and VEGF-negative. The rest of PCCs in CNVMs were clearly Cytokeratine-negative and were separated CD68-negative cells from CD68- positive cells. CD68-negative cells were VEGF-positive and located on the suface of CNVMs. CD68-positive cells were VEGF-negative and seen adjacent to the RPE layer or CD68-positive cells. RPE in CNVMs may loose characteristics of epithelium and perhaps become VEGF-positive or CD68-positive according to their roles.

Keywords: age-related macular degeneration • retinal pigment epithelium • choroid: neovascularization 
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