May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Morphologically Different Intrinsically Photosensitive Ganglion Cells (ipRGCs) Have Different Ca Responses to Light
Author Affiliations & Notes
  • K. Balint
    Neurobiology, Friedrich Miescher Institute, Basel, Switzerland
    Institute of Biology, University of Szeged, Szeged, Hungary
  • D. Balya
    Neurobiology, Friedrich Miescher Institute, Basel, Switzerland
  • Z. Boldogkoi
    Institute of Biology, University of Szeged, Szeged, Hungary
  • B. Roska
    Neurobiology, Friedrich Miescher Institute, Basel, Switzerland
  • Footnotes
    Commercial Relationships  K. Balint, None; D. Balya, None; Z. Boldogkoi, None; B. Roska, None.
  • Footnotes
    Support  Novartis Research Foundation, Marie Curie Excellence Grant
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1410. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      K. Balint, D. Balya, Z. Boldogkoi, B. Roska; Morphologically Different Intrinsically Photosensitive Ganglion Cells (ipRGCs) Have Different Ca Responses to Light. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1410. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Our goal was to investigate the light induced Ca dynamics of morphologically identified subclasses of ipRGCs.

Methods: : We created a recombinant Pseudorabies virus (PRV) based, retrograde, transsynaptic tracer expressing a genetically encoded Ca-sensor. This, Activity-sensor PRV (As-PRV) was injected into the anterior chamber of the right eye of adult mice. 3.5-5 days after the injections the contralateral retina was isolated and stimulated with light. Ca-imaging was performed from labeled ganglion cells with the help of 2-photon microscopy. After live-imaging, the retinas were fixed and stained with different antibodies for further analysis via confocal microscopy.

Results: : As-PRV infects highly specifically the pupillary reflex pathway including the Edinger-Westphal and Olivary pretectal nuclei as well as other connected regions like the Intergeniculate leaflet or Suprachiasmatic nucleus. In the contralateral retina As-PRV labeled mainly ipRGCs at early times of retinal infection. Light stimulation of the retina resulted in large Ca changes in a well defined morphological class of ipRGCs while another class of ipRGCs had much smaller or undetectable Ca response. Morphological analysis of the recorded cells showed, that the cells with large Ca response were stratifying in the OFF sublamina while the other class stratified in the ON sublamina.

Conclusions: : Our results indicate that different morphological classes of ipRGCs have remarkably different Ca responses during light activation. These ipRGC subclasses might activate different brain regions or different subtypes of neurons in ipRGC target regions.

Keywords: retinal connections, networks, circuitry • calcium 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×