May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Redox Balance in the Lens After in vivo Exposure to UVR, Dependence of Alpha-Tocopherol Supplementation
Author Affiliations & Notes
  • J. Wang
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
    Department of Ophthalmology, Shandong University, Jinan, China
  • S. Löfgren
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • X. Dong
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • K. Galichanin
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • V. Mody, Jr.
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • M. Ayala
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • L. Meyer
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
    Dept. of Ophthalmology, University of Bonn, Bonn, Germany
  • P. Söderberg
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • Footnotes
    Commercial Relationships  J. Wang, None; S. Löfgren, None; X. Dong, None; K. Galichanin, None; V. Mody, None; M. Ayala, None; L. Meyer, None; P. Söderberg, None.
  • Footnotes
    Support  Karolinska Institutet Research Foundation, Swedish Council for Working Life and Social Research, Swedish Research Council, Carmen och Bertil Regners Stiftelsen
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1465. doi:https://doi.org/
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      J. Wang, S. Löfgren, X. Dong, K. Galichanin, V. Mody, Jr., M. Ayala, L. Meyer, P. Söderberg; Redox Balance in the Lens After in vivo Exposure to UVR, Dependence of Alpha-Tocopherol Supplementation. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1465. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the redox balance in the albino rat lens as a function of dose of oral supplementation of alpha-tocopherol dose after in vivo exposure to ultraviolet radiation (UVR).

Methods: : Five groups of twenty, six-week-old female albino Sprague Dawley rats were for four weeks supplemented with 0, 5, 25, 50, and 100 IU/day alpha-tocopherol orally depending on group belonging. Then, each rat was unilaterally exposed in vivo to 8 kJ/m2 (exp time 15 min ) UVR-300 nm. The rats were sacrificed at 1 week after exposure to UVR and the lenses were extracted and homogenized. Lens total reduced (GSH) and oxidized (GSSG) glutathione; glutathione- reductase (GR) and oxidase (GPx) were determined colorimetrically by enzyme reactions.

Results: : UVR induced light scattering was dose dependently protected with Alpha-tocopherol with a rate constant (1/e) of 4.8 IU/day. UVR exposure cased a depletion of total lens GSH at low alpha-tocopherol supplementation that recovered towards higher alpha-tocopherol supplementation but did not cause any alteration of total lens GSSG at any level of alpha-tocopherol supplementation. No alteration of total lens GR- or GPx activity at any level of alpha-tocopherol supplementation was observed after UVR exposure.

Conclusions: : Alpha-tocopherol protects against UVR induced light scattering, dose dependently. The protection is associated with a reduction of GSH depletion caused by UVR exposure without any effect on total lens GSSG or GR- or GPx activity.

Keywords: radiation damage: light/UV • cataract • antioxidants 
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