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T. Hattori, Y. Usui, N. Yamakawa, Y. Okunuki, H. Akiba, M. Takiuchi, H. Goto; Functional Expression of CD40 in Corneal Neovasculization. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1480. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
CD40 is one of the co-stimulatory molecules, and interacts with its ligand, CD40L expressed on activate T lymphocytes. Recent study demonstrated that not only lymphocytes but also vascular endothelial cells express CD40. In this study, we investigated whether costimulatory signals via CD40/CD40L pathway were involved in corneal angiogenesis using the mouse model of suture-induced corneal neovascularization.
One suture with 10-0 nylon was placed intrastromally and suture was left in place for 7 days. Seven days after the placement, the corneas were excised and analyzed for expression of CD40 and CD40L by RT-PCR and immunohistochemistry. Some groups of mice received agonistic anti-CD40 mAb (FGK45) intraperitoneally on day 0, 3, and 5 after the suture placement. Control mice received equal volume of rat isotype IgG. In addition, anti-lectin-FITC antibody was injected intravenously 7 days after suture placement, and extension of blood vessels on the cornea was numerically measured using software of NIH image.
Expression of CD40 was observed in the vascularized corneas but not in intact corneas, which was also verified by mRNA expression of CD40. Administration of agonistic anti-CD40 mAb significantly progressed corneal neovascularization compared with control Rat IgG treatment. On the other hand, expression of CD40L was not observed in the vascularized corneas by either RT-PCR nor immunohistochemistry.
CD40 molecule is expressed on the vasculized cornea, which delivers costimulatory signals involved in progression of corneal neovascularization.
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