May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Functional Expression of CD40 in Corneal Neovasculization
Author Affiliations & Notes
  • T. Hattori
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • Y. Usui
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • N. Yamakawa
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • Y. Okunuki
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • H. Akiba
    Immunology, Juntendo University, Bunkyo-ku, Japan
  • M. Takiuchi
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • H. Goto
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • Footnotes
    Commercial Relationships  T. Hattori, None; Y. Usui, None; N. Yamakawa, None; Y. Okunuki, None; H. Akiba, None; M. Takiuchi, None; H. Goto, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1480. doi:https://doi.org/
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    • Get Citation

      T. Hattori, Y. Usui, N. Yamakawa, Y. Okunuki, H. Akiba, M. Takiuchi, H. Goto; Functional Expression of CD40 in Corneal Neovasculization. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1480. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : CD40 is one of the co-stimulatory molecules, and interacts with its ligand, CD40L expressed on activate T lymphocytes. Recent study demonstrated that not only lymphocytes but also vascular endothelial cells express CD40. In this study, we investigated whether costimulatory signals via CD40/CD40L pathway were involved in corneal angiogenesis using the mouse model of suture-induced corneal neovascularization.

Methods: : One suture with 10-0 nylon was placed intrastromally and suture was left in place for 7 days. Seven days after the placement, the corneas were excised and analyzed for expression of CD40 and CD40L by RT-PCR and immunohistochemistry. Some groups of mice received agonistic anti-CD40 mAb (FGK45) intraperitoneally on day 0, 3, and 5 after the suture placement. Control mice received equal volume of rat isotype IgG. In addition, anti-lectin-FITC antibody was injected intravenously 7 days after suture placement, and extension of blood vessels on the cornea was numerically measured using software of NIH image.

Results: : Expression of CD40 was observed in the vascularized corneas but not in intact corneas, which was also verified by mRNA expression of CD40. Administration of agonistic anti-CD40 mAb significantly progressed corneal neovascularization compared with control Rat IgG treatment. On the other hand, expression of CD40L was not observed in the vascularized corneas by either RT-PCR nor immunohistochemistry.

Conclusions: : CD40 molecule is expressed on the vasculized cornea, which delivers costimulatory signals involved in progression of corneal neovascularization.

Keywords: neovascularization • cornea: basic science 
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