May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Effects of Resveratrol on Trabecular Meshwork (TM) Cells Exposed to Chronic Oxidative Stress
Author Affiliations & Notes
  • C. C. Luna
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • G. Li
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • P. B. Liton
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • P. Challa
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • D. L. Epstein
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • P. Gonzalez
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Footnotes
    Commercial Relationships  C.C. Luna, None; G. Li, None; P.B. Liton, None; P. Challa, None; D.L. Epstein, None; P. Gonzalez, None.
  • Footnotes
    Support  NEI EY01894, NEI EY016228, NEI EY05722, and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1624. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      C. C. Luna, G. Li, P. B. Liton, P. Challa, D. L. Epstein, P. Gonzalez; Effects of Resveratrol on Trabecular Meshwork (TM) Cells Exposed to Chronic Oxidative Stress. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1624. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To evaluate the effects of resveratrol on the expression of markers for inflammation, oxidative damage, and cellular senescence in primary trabecular meshwork (TM) cells subjected to chronic oxidative stress.

Methods: : Porcine TM cells where subjected to chronic oxidative stress by incubation in hyperoxic conditions (40% oxygen) for 15 days with resveratrol (25 µM) or vehicle (DMSO) and compared to cells incubated at 5% oxygen. The potential protective effects of resveratrol treatment were evaluated by measurements of the following parameters: expression of inflammatory markers (IL1alpha, IL6, IL8, and ELAM-1), autofluorescence, production of intracellular ROS, senescence associated beta-galactosidase (sa-beta-gal) activity, DNA damage, proteasome activity, protein carbonilation, cell proliferation, and apoptosis.

Results: : Treatment with resveratrol effectively prevented the increased production of iROS as well as the upregulation of inflammatory markers IL1alpha, IL6, IL8, and ELAM-1 after chronic oxidative stress. Resveratrol treatment also resulted in reduced expression of the senescence markers sa-beta-gal, lipofucsin and carbonilated proteins. Furthermore, resveratrol exerted antiapoptotic effects in an acute oxidative stress model. These effects were not associated with a decrease in cell proliferation.

Conclusions: : The inhibition of iROS production by resveratrol may help to prevent the induction of inflammatory and senescence markers in TM cells after chronic oxidative stress. These results together with the observed anti-apoptotic effects suggest that resveratrol could potentially have a novel therapeutic role in preventing the TM tissue abnormalities observed in POAG.

Keywords: trabecular meshwork • oxidation/oxidative or free radical damage • drug toxicity/drug effects 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×