May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Myopic & Hyperopic Defocus Effects on Chick Scleral MMP-2, MMP-13, TGFß-2, and TIMP-2 mRNA Levels
Author Affiliations & Notes
  • J. Su
    Vision Science, University of California - Berkeley, Berkeley, California
  • Z. Zhang
    Vision Science, University of California - Berkeley, Berkeley, California
  • C. Wildsoet
    Vision Science, University of California - Berkeley, Berkeley, California
  • Footnotes
    Commercial Relationships  J. Su, None; Z. Zhang, None; C. Wildsoet, None.
  • Footnotes
    Support  NIH EY-R012392
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1740. doi:https://doi.org/
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    • Get Citation

      J. Su, Z. Zhang, C. Wildsoet; Myopic & Hyperopic Defocus Effects on Chick Scleral MMP-2, MMP-13, TGFß-2, and TIMP-2 mRNA Levels. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1740. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

Based on previous findings of visually-induced transcriptional regulation of various proteins in the chick sclera1, we sought to investigate further the effects of myopic and hyperopic defocus on the mRNA expression levels of gelatinase-A (MMP-2), collagenase 3 (MMP-13), transforming growth factor beta-2 (TGFß-2), and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2), which are implicated in scleral remodeling and have the potential to be used to tailor novel biomaterials to treat high myopia.

 
Methods:
 

Myopic or hyperopic defocus was imposed on White-Leghorn chicks (3 wks old) using -10 or +10D lenses for 4 & 48h. Fellow eyes were left untreated as controls. Scleral layers were harvested and separated in the afternoon, and relative mRNA levels of MMP-2, MMP-13, TIMP-2 and TGFß-2 were measured by real-time quantitative PCR. Statistical significance was computed by paired student’s t-test.

 
Results:
 

Significant changes in mRNA expression were observed for all 4 proteins, albeit not at all time points. Typically, the results for fibrous and cartilaginous layers showed opposite trends as did results for (+) and (-) lenses (Table 1). Expression levels in the fellow eyes of the 2 lens groups were not significantly different, i.e. there was no interocular yoking.Table 1. Increased (>), decreased (<), or similar (-) scleral mRNA expression in lens-treated vs fellow eyes at 4 or 48h. (*p < 0.001).  

 
Conclusions:
 

As reported by Schippert et al1, TGFß-2 expression showed an early increase with (+) lenses in both scleral layers, similar to our finding in retinal pigment epithelium after 48h lens wear, and consistent with a role as an inhibitory growth modulator. However, the transient nature of its upregulation suggests additional growth modulatory influences on the latter. The cartilage expression patterns for MMP-2 & -13 are consistent with the opposite directions of growth induced by (+) and (-) lenses, only TIMP-2 showed robust expression changes in fibrous sclera. Their suitability as targets for biomaterials to treat high myopia requires verification of such changes after long-term treatment.1Schippert, R., et al., 2006. Exp Eye Res, 82:710-719.

 
Keywords: myopia • gene/expression 
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